B6;129P2-Gja1tm4Kwi/Cnrm

Status

Available to order

EMMA IDEM:01643
International strain nameB6;129P2-Gja1tm4Kwi/Cnrm
Alternative nameCx43KICx43K258stop
Strain typeTargeted Mutant Strains : Knock-in
Allele/Transgene symbolGja1tm4Kwi,
Gene/Transgene symbolGja1

Information from provider

ProviderKlaus WILLECKE
Provider affiliationMolekulargenetik, Institut fuer Genetik, Universitaet Bonn
Genetic informationInstead of the whole open reading frame of exon 2 of the Gja1 (Cx43) gene , a truncated form of Gja1 (leading to a deletion of the last 125 aminoacids) is expressed, under the control of the endogenous Gja1 promoter.
Phenotypic informationDefects in the barrier function of the epidermis, dilatation of the heart, impaired folliculogenesis.
Breeding historyAfter blastocyst injection the chimeras were bred with C57BL/6NCrl to obtain brown coat coloured offspring. This brown offspring was backcrossed to C57BL/6NCrl more than 2 times.
References
  • Defective epidermal barrier in neonatal mice lacking the C-terminal region of connexin43.;Maass Karen, Ghanem Alexander, Kim Jung-Sun, Saathoff Manuela, Urschel Stephanie, Kirfel Gregor, Grümmer Ruth, Kretz Markus, Lewalter Thorsten, Tiemann Klaus, Winterhager Elke, Herzog Volker, Willecke Klaus, ;2004;Molecular biology of the cell;15;4597-608; 15282340

Information from EMMA

Archiving centreCNR, Consiglio Nazionale delle Ricerche, Monterotondo, Italy
Breeding at archiving centreBackcrossed to C57BL/6J

Disease and phenotype information

Orphanet associated rare diseases, based on orthologous gene matching

MGI phenotypes (allele matching)
  • integument phenotype / MGI
  • postnatal lethality, incomplete penetrance / MGI
  • abnormal heart shape / MGI
  • abnormal ovarian follicle morphology / MGI
  • absent mature ovarian follicles / MGI
  • absent corpus luteum / MGI
  • abnormal skin condition / MGI
  • abnormal epidermis stratum granulosum morphology / MGI
  • abnormal epidermis stratum corneum morphology / MGI
  • female infertility / MGI
  • small heart / MGI
  • dilated heart left ventricle / MGI
  • impaired skin barrier function / MGI
  • prolonged QT interval / MGI
  • impaired luteinization / MGI
  • decreased heart rate / MGI

Literature references

  • Defective epidermal barrier in neonatal mice lacking the C-terminal region of connexin43.;Maass Karen, Ghanem Alexander, Kim Jung-Sun, Saathoff Manuela, Urschel Stephanie, Kirfel Gregor, Grümmer Ruth, Kretz Markus, Lewalter Thorsten, Tiemann Klaus, Winterhager Elke, Herzog Volker, Willecke Klaus, ;2004;Molecular biology of the cell;15;4597-608; 15282340

Information on how we integrate external resources can be found here

Order

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

  • Frozen embryos. Delivered in 4 weeks (after paperwork in place). €1740*
  • Rederivation of mice from frozen stock, delivery time available upon request . €3880*

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
For this strain no provider MTA is needed. Distribution is based on the EMMA conditions only.

EMMA conditions
Legally binding conditions for the transfer

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