B6.129P2-Gjd2tm2Kwi/Cnrm
| Status | Available to order |
| EMMA ID | EM:01646 |
| International strain name | B6.129P2-Gjd2tm2Kwi/Cnrm |
| Alternative name | Cx36 fl |
| Strain type | Targeted Mutant Strains : Knock-out |
| Allele/Transgene symbol | Gjd2tm2Kwi, |
| Gene/Transgene symbol | Gjd2 |
Information from provider
| Provider | Klaus WILLECKE |
| Provider affiliation | Molekulargenetik, Institut fuer Genetik, Universitaet Bonn |
| Genetic information | Instead of exon 2 of Gjd2 (Cx36), a loxP sites-flanked construct is inserted; it contains the exon 2 of Gjd2 and a frt-sites-flanked neomycin selection cassette. After cre recombinase activity a lacZ reporter gene is expressed under the control of the endogenous Gjd2 promoter. |
| Phenotypic information | Like Gjd2 knock-out mice (because of no expression of Gjd2 in the loxP-flanked state): visual transmission defect, reduced gamma oscillation. |
| Breeding history | After blastocyst injection the chimeras were bred with C57BL/6NCrl to obtain brown coat colored offspring. This brown offspring was backcrossed to C57BL/6NCrl more than 5 times. |
| References |
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Information from EMMA
| Archiving centre | CNR, Consiglio Nazionale delle Ricerche, Monterotondo, Italy |
| Breeding at archiving centre | Backcrossed to C57BL/6J |
Literature references
- Expression pattern of lacZ reporter gene representing connexin36 in transgenic mice.;Degen Joachim, Meier Carola, Van Der Giessen Ruben S, Söhl Goran, Petrasch-Parwez Elisabeth, Urschel Stephanie, Dermietzel Rolf, Schilling Karl, De Zeeuw Chris I, Willecke Klaus, ;2004;The Journal of comparative neurology;473;511-25; 15116387