B6;129P2-Gjc1tm1Weil/Cnrm
| Status | Available to order |
| EMMA ID | EM:01647 |
| International strain name | B6;129P2-Gjc1tm1Weil/Cnrm |
| Alternative name | Cx45 flox |
| Strain type | Targeted Mutant Strains : Conditional mutation |
| Allele/Transgene symbol | Gjc1tm1Weil, |
| Gene/Transgene symbol | Gjc1 |
Information from provider
| Provider | Klaus WILLECKE |
| Provider affiliation | Molekulargenetik, Institut fuer Genetik, Universitaet Bonn |
| Genetic information | Instead of the whole open reading frame of exon 3 of the Gjc1 (Cx45) gene, a loxP sites-flanked construct was inserted, containing the exon 3 of Gjc1. An frt-sites-flanked neomycin-selection cassette was placed in intron 2. After cre recombinase activity an eGFP reporter gene is expressed under the control of the endogenous Gjc1 promoter. |
| Phenotypic information | None (loxP-flanked gene mutation). |
| Breeding history | After blastocyst injection the chimeras were bred with C57BL/6NCrl to obtain brown coat coloured offspring. This brown offspring was backcrossed to C57BL/6NCrl more than 3 times. |
| References |
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Information from EMMA
| Archiving centre | CNR, Consiglio Nazionale delle Ricerche, Monterotondo, Italy |
| Breeding at archiving centre | Backcrossed to C57BL/6J |
Literature references
- Deletion of connexin45 in mouse retinal neurons disrupts the rod/cone signaling pathway between AII amacrine and ON cone bipolar cells and leads to impaired visual transmission.;Maxeiner Stephan, Dedek Karin, Janssen-Bienhold Ulrike, Ammermüller Josef, Brune Hendrik, Kirsch Taryn, Pieper Mario, Degen Joachim, Krüger Olaf, Willecke Klaus, Weiler Reto, ;2005;The Journal of neuroscience : the official journal of the Society for Neuroscience;25;566-76; 15659592