C57BL/6N-Mbd5^{tm1b(EUCOMM)Wtsi}/Ics

Status	Under development - register interest
EMMA ID	EM:16694
Citation information	RRID:IMSR_EM:16694 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information.
International strain name	C57BL/6N-Mbd5^{tm1b(EUCOMM)Wtsi}/Ics
Alternative name	EPD0056_1_E03
Strain type	Targeted Mutant Strains
Allele/Transgene symbol	Mbd5^{tm1b(EUCOMM)Wtsi}
Gene/Transgene symbol	Mbd5
Disclaimer	Please note that for EUCOMM and KOMP-CSD mice supplied to the scientific community by INFRAFRONTIER/EMMA: We can not guarantee a null mutation for Knock-out first alleles (tm1a alleles, see http://www.mousephenotype.org/about-ikmc/targeting-strategies) as the critical exon has not been deleted. That the structure of the targeted mutation in the ES cells obtained from EUCOMM/KOMP to generate EUCOMM/KOMP mice is not verified by INFRAFRONTIER/EMMA. It is recommended that the recipient confirms the mutation structure. No check for determining the copy number of the targeting construct in ES cells obtained from EUCOMM/KOMP is done by INFRAFRONTIER/EMMA. The level of quality control before mice are released is to confirm the individual mouse genotype by short range PCR.

Information from provider

Provider	ICS, Institut Clinique de la Souris
Provider affiliation	ICS, Institut Clinique de la Souris
Genetic information	This mouse line originates from EUCOMM ES clone EPD0056_1_E03. For further details on the construction of this clone see the page at the IMPC portal. The critical exon(s) were flanked by loxP sites, and subsequent cre expression excised this critical sequence resulting in a knockout reporter allele (Gt(ROSA)26Sortm1(ACTB-cre,-EGFP)Ics). Click here for more information on EUCOMM final vectors.
Phenotypic information	Potential phenotyping data in the IMPC portal
References	None available

Information from EMMA

Archiving centre	ICS, Institut Clinique de la Souris, Illkirch-Graffenstaden, France

Disease and phenotype information

Orphanet associated rare diseases, based on orthologous gene matching

Autosomal dominant non-syndromic intellectual disability / Orphanet_178469

IMPC phenotypes (allele matching)

decreased leukocyte cell number / IMPC
decreased locomotor activity / IMPC
decreased exploration in new environment / IMPC
short tibia / IMPC
abnormal lip morphology / IMPC
decreased lymphocyte cell number / IMPC
preweaning lethality, complete penetrance / IMPC
decreased total retina thickness / IMPC

IMPC phenotypes (gene matching)

decreased locomotor activity / IMPC
decreased leukocyte cell number / IMPC
short tibia / IMPC
decreased total retina thickness / IMPC
abnormal lip morphology / IMPC
decreased lymphocyte cell number / IMPC
preweaning lethality, complete penetrance / IMPC
decreased exploration in new environment / IMPC

MGI phenotypes (gene matching)

abnormal nasal bone morphology / MGI
hypoglycemia / MGI
decreased body weight / MGI
decreased body size / MGI
abnormal social investigation / MGI
impaired coordination / MGI
increased grooming behavior / MGI
postnatal growth retardation / MGI
abnormal learning/memory/conditioning / MGI
abnormal motor capabilities/coordination/movement / MGI
abnormal glucose homeostasis / MGI
increased brain weight / MGI
decreased circulating insulin level / MGI
increased insulin sensitivity / MGI
decreased liver weight / MGI
abnormal response to novel object / MGI
decreased circulating insulin-like growth factor I level / MGI
decreased growth hormone level / MGI
cachexia / MGI
improved glucose tolerance / MGI
small pituitary gland / MGI
behavior/neurological phenotype / MGI
decreased circulating glucose level / MGI
abnormal neurite morphology / MGI
decreased subcutaneous adipose tissue amount / MGI
decreased abdominal adipose tissue amount / MGI
decreased gonadal fat pad weight / MGI
decreased grip strength / MGI
integument phenotype / MGI
postnatal lethality, complete penetrance / MGI
perinatal lethality, incomplete penetrance / MGI

Information on how we integrate external resources can be found here

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

Frozen sperm. Delivered in 4 weeks (after paperwork in place). €1740^*

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

^* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
Distribution of this strain is subject to a provider MTA. Both signing of the MTA and submission of the online EMMA Mutant Request Form are required before material can be shipped.

EMMA conditions
Legally binding conditions for the transfer

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C57BL/6N-Mbd5tm1b(EUCOMM)Wtsi/Ics