B6Dnk;B6Brd;B6N-Tyrc-Brd Cishtm1a(KOMP)Wtsi/WtsiCnbc

Status

Under development - register interest

EMMA IDEM:16728
Citation informationRRID:IMSR_EM:16728 

Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information.

 
International strain nameB6Dnk;B6Brd;B6N-Tyrc-Brd Cishtm1a(KOMP)Wtsi/WtsiCnbc
Alternative nameEPD0165_3_H07
Strain typeTargeted Mutant Strains
Allele/Transgene symbolCishtm1a(KOMP)Wtsi
Gene/Transgene symbolCish
DisclaimerPlease note that for EUCOMM and KOMP-CSD mice supplied to the scientific community by INFRAFRONTIER/EMMA:
  1. We can not guarantee a null mutation for Knock-out first alleles (tm1a alleles, see http://www.mousephenotype.org/about-ikmc/targeting-strategies) as the critical exon has not been deleted.
  2. That the structure of the targeted mutation in the ES cells obtained from EUCOMM/KOMP to generate EUCOMM/KOMP mice is not verified by INFRAFRONTIER/EMMA. It is recommended that the recipient confirms the mutation structure.
  3. No check for determining the copy number of the targeting construct in ES cells obtained from EUCOMM/KOMP is done by INFRAFRONTIER/EMMA.
  4. The level of quality control before mice are released is to confirm the individual mouse genotype by short range PCR.

Information from provider

Provider Wellcome Trust Sanger Institute
Provider affiliationWellcome Trust Sanger Institute
Genetic informationThis mouse line originates from KOMP ES clone EPD0165_3_H07. For further details on the construction of this clone see the page at the IMPC portal.
Phenotypic informationPotential phenotyping data in the IMPC portal
ReferencesNone available

Information from EMMA

Archiving centreCNB-CSIC, Centro Nacional de Biotecnologia, Madrid, Spain

Disease and phenotype information

IMPC phenotypes (allele matching)
  • increased circulating chloride level / IMPC
IMPC phenotypes (gene matching)
  • increased circulating chloride level / IMPC
MGI phenotypes (gene matching)
  • abnormal immune system morphology / MGI
  • spleen hyperplasia / MGI
  • thymus hyperplasia / MGI
  • abnormal immune system physiology / MGI
  • lung inflammation / MGI
  • abnormal respiratory mucosa goblet cell morphology / MGI
  • increased eosinophil cell number / MGI
  • increased lymphocyte cell number / MGI
  • increased T cell proliferation / MGI
  • immune system phenotype / MGI
  • increased susceptibility to type I hypersensitivity reaction / MGI
  • pulmonary fibrosis / MGI
  • abnormal CD4-positive T cell differentiation / MGI
  • abnormal T-helper 2 cell differentiation / MGI
  • lymph node hyperplasia / MGI
  • increased interleukin-13 secretion / MGI
  • increased interleukin-4 secretion / MGI
  • increased interleukin-5 secretion / MGI
  • increased interleukin-9 secretion / MGI

Information on how we integrate external resources can be found here

Register interest

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

  • Frozen sperm. Delivered in 4 weeks (after paperwork in place). €1740*

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
Distribution of this strain is subject to a provider MTA. Both signing of the MTA and submission of the online EMMA Mutant Request Form are required before material can be shipped.

EMMA conditions
Legally binding conditions for the transfer

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