IMPC phenotypes (gene matching)
B6-Cpa3tm3(icre)Hrr
| Status | Under development - register interest |
| EMMA ID | EM:16832 |
| Citation information | RRID:IMSR_EM:16832 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information. |
| International strain name | B6-Cpa3tm3(icre)Hrr |
| Alternative name | B6-Cpa3tm3(icre)Hrr |
| Strain type | Targeted Mutant Strains : Knock-in |
| Allele/Transgene symbol | Cpa3tm3(icre)Hrr |
| Gene/Transgene symbol | Cpa3 |
Information from provider
| Provider | Hans-Reimer Rodewald |
| Provider affiliation | Cellular Immunology D110, German Cancer Research Center |
| Additional owner | Dr. Thorsten Feyerabend. German Cancer Research Center. Cellular Immunology D110. Heidelberg, Germany. |
| Genetic information | In the first exon of the Cpa3 gene, the 28 nucleotides coding for the first 9 amino acids were replaced with a codon-improved cre recombinase (icre) expression cassette and an FRT-flanked neomycin selection cassette. The cre recombinase expression cassette consists of a splice donor, intron, and splice acceptor from rabbit beta-globin, and the codon-improved cre sequence (Shimshek et al., Genesis. 2002; doi:10.1002/gene.10023), followed by the rabbit beta-globin polyadenylation site. Transient expression of flp recombinase in a clone of correctly targeted ES cells removed the selection cassette. br bPlease note:/b According to the information provided by the depositor and the submitted material, this strain shares the same targeted allele as the related EMMA strain (EM:16833) but differs in genetic background. The strains can be visually distinguished by their coat colour. Where the distinction between the strains is relevant for experimental use, recipients are advised to confirm the genetic background using appropriate strain-specific genetic markers (e.g. SNP analysis). |
| Phenotypic information | Homozygous:No additional phenotype was observed compared with heterozygous mice.Heterozygous:Heterozygous mice are specifically deficient in mast cells. Mucosal and connective tissue mast cells are absent. Basophil numbers are reduced. The cre recombinase is expressed in mast cell and basophil lineage (MCBP) as well as in thymic progenitors (ETP and DN2). |
| Breeding history | More than 20 times backcrossed to C57BL/6J. The strain is maintained by HET × WT brother–sister mating. |
| References |
|
| Homozygous fertile | yes |
| Homozygous viable | yes |
| Homozygous matings required | no |
| Immunocompromised | no |
Information from EMMA
| Archiving centre | Helmholtz Zentrum Muenchen - German Research Center for Environmental Health (GmbH), Oberschleißheim, Germany |
| Animals used for archiving | heterozygous C57BL/6J males |
Disease and phenotype information
Literature references
- Cre-mediated cell ablation contests mast cell contribution in models of antibody- and T cell-mediated autoimmunity.;Feyerabend Thorsten B, Weiser Anne, Tietz Annette, Stassen Michael, Harris Nicola, Kopf Manfred, Radermacher Peter, Möller Peter, Benoist Christophe, Mathis Diane, Fehling Hans Jörg, Rodewald Hans-Reimer, ;2011;Immunity;35;832-44; 22101159
- Widespread immunological functions of mast cells: fact or fiction?;Rodewald Hans-Reimer, Feyerabend Thorsten B, ;2012;Immunity;37;13-24; 22840840
- Mast cells are dispensable for normal and activin-promoted wound healing and skin carcinogenesis.;Antsiferova Maria, Martin Caroline, Huber Marcel, Feyerabend Thorsten B, Förster Anja, Hartmann Karin, Rodewald Hans-Reimer, Hohl Daniel, Werner Sabine, ;2013;Journal of immunology (Baltimore, Md. : 1950);191;6147-55; 24227781
- Mast cells play no role in the pathogenesis of postoperative ileus induced by intestinal manipulation.;Gomez-Pinilla Pedro J, Farro Giovanna, Di Giovangiulio Martina, Stakenborg Nathalie, Némethova Andrea, de Vries Annick, Liston Adrian, Feyerabend Thorsten B, Rodewald Hans-Reimer, Boeckxstaens Guy E, Matteoli Gianluca, ;2014;PloS one;9;e85304; 24416383
- Type 1 diabetes in NOD mice unaffected by mast cell deficiency.;Gutierrez Dario A, Fu Wenxian, Schonefeldt Susann, Feyerabend Thorsten B, Ortiz-Lopez Adriana, Lampi Yulia, Liston Adrian, Mathis Diane, Rodewald Hans-Reimer, ;2014;Diabetes;63;3827-34; 24917576
- A next-generation dual-recombinase system for time- and host-specific targeting of pancreatic cancer.;Schönhuber Nina, Seidler Barbara, Schuck Kathleen, Veltkamp Christian, Schachtler Christina, Zukowska Magdalena, Eser Stefan, Feyerabend Thorsten B, Paul Mariel C, Eser Philipp, Klein Sabine, Lowy Andrew M, Banerjee Ruby, Yang Fangtang, Lee Chang-Lung, Moding Everett J, Kirsch David G, Scheideler Angelika, Alessi Dario R, Varela Ignacio, Bradley Allan, Kind Alexander, Schnieke Angelika E, Rodewald Hans-Reimer, Rad Roland, Schmid Roland M, Schneider Günter, Saur Dieter, ;2014;Nature medicine;20;1340-1347; 25326799
- Hematopoietic Kit Deficiency, rather than Lack of Mast Cells, Protects Mice from Obesity and Insulin Resistance.;Gutierrez Dario A, Muralidhar Sathya, Feyerabend Thorsten B, Herzig Stephan, Rodewald Hans-Reimer, ;2015;Cell metabolism;21;678-91; 25955205
- Mast cells are dispensable in a genetic mouse model of chronic dermatitis.;Sulcova Jitka, Meyer Michael, Guiducci Eva, Feyerabend Thorsten B, Rodewald Hans-Reimer, Werner Sabine, ;2015;The American journal of pathology;185;1575-87; 25843682
- Mast cells have no impact on cutaneous leishmaniasis severity and related Th2 differentiation in resistant and susceptible mice.;Paul Christoph, Wolff Svenja, Zapf Thea, Raifer Hartmann, Feyerabend Thorsten B, Bollig Nadine, Camara Bärbel, Trier Claudia, Schleicher Ulrike, Rodewald Hans-Reimer, Lohoff Michael, ;2016;European journal of immunology;46;114-21; 26449668
- Mucosal mast cells are indispensable for the timely termination of Strongyloides ratti infection.;Reitz M, Brunn M-L, Rodewald H-R, Feyerabend T B, Roers A, Dudeck A, Voehringer D, Jönsson F, Kühl A A, Breloer M, ;2017;Mucosal immunology;10;481-492; 27381924
- Unimpaired Responses to Vaccination With Protein Antigen Plus Adjuvant in Mice With Kit-Independent Mast Cell Deficiency.;Schubert Nadja, Lisenko Katharina, Auerbach Christian, Weitzmann Anke, Ghouse Shanawaz Mohammed, Muhandes Lina, Haase Christa, Häring Tobias, Schulze Livia, Voehringer David, Gunzer Florian, Müller Werner, Feyerabend Thorsten B, Rodewald Hans-Reimer, Dudeck Anne, Roers Axel, ;2018;Frontiers in immunology;9;1870; 30210490
- Human Mast Cell Proteome Reveals Unique Lineage, Putative Functions, and Structural Basis for Cell Ablation.;Plum Thomas, Wang Xi, Rettel Mandy, Krijgsveld Jeroen, Feyerabend Thorsten B, Rodewald Hans-Reimer, ;2020;Immunity;52;404-416.e5; 32049054
- Mast cells link immune sensing to antigen-avoidance behaviour.;Plum Thomas, Binzberger Rebecca, Thiele Robin, Shang Fuwei, Postrach Daniel, Fung Candice, Fortea Marina, Stakenborg Nathalie, Wang Zheng, Tappe-Theodor Anke, Poth Tanja, MacLaren Duncan A A, Boeckxstaens Guy, Kuner Rohini, Pitzer Claudia, Monyer Hannah, Xin Cuiyan, Bonventre Joseph V, Tanaka Satoshi, Voehringer David, Vanden Berghe Pieter, Strid Jessica, Feyerabend Thorsten B, Rodewald Hans-Reimer, ;2023;Nature;620;634-642; 37438525
- Beyond classical immunity: Mast cells as signal converters between tissues and neurons.;Plum Thomas, Feyerabend Thorsten B, Rodewald Hans-Reimer, ;2024;Immunity;57;2723-2736; 39662090