IMPC phenotypes (gene matching)
C-Cpa3tm3(icre)Hrr
| Status | Under development - register interest |
| EMMA ID | EM:16833 |
| Citation information | RRID:IMSR_EM:16833 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information. |
| International strain name | C-Cpa3tm3(icre)Hrr |
| Alternative name | C-Cpa3tm3(icre)HRR |
| Strain type | Targeted Mutant Strains : Knock-in |
| Allele/Transgene symbol | Cpa3tm3(icre)Hrr |
| Gene/Transgene symbol | Cpa3 |
Information from provider
| Provider | Hans-Reimer Rodewald |
| Provider affiliation | Cellular Immunology D110, German Cancer Research Center |
| Additional owner | Dr. Thorsten Feyerabend. German Cancer Research Center. Cellular Immunology D110. Heidelberg, Germany. |
| Genetic information | In the first exon of the Cpa3 gene, the 28 nucleotides coding for the first 9 amino acids were replaced with a codon-improved cre recombinase expression cassette and a Frt-flanked neomycin selection cassette. The cre recombinase expression cassette consists of a splice donor, intron, splice acceptor from rabbit beta-globin and the codon-improved cre sequence (Shimshek et al., Genesis. 2002; doi:10.1002/gene.10023), followed by the rabbit beta-globin polyadenylation site. Transient expression of flp recombinase in a clone of correctly targeted ES cells removed the selection cassette. br bPlease note:/b According to the information provided by the depositor and the submitted material, this strain shares the same targeted allele as the related EMMA strain (EM:16832) but differs in genetic background. The strains can be visually distinguished by their coat colour. Where the distinction between the strains is relevant for experimental use, recipients are advised to confirm the genetic background using appropriate strain-specific genetic markers (e.g. SNP analysis). |
| Phenotypic information | Homozygous:No additional phenotype known compared to heterozygous mice.Heterozygous:Heterozygous mice are specifically mast cell deficient. Mucosal and connective tissue mast cells are absent. Reduction of basophils. The cre recombinase is expressed in progenitors of the mast cell and basophil lineage (MCBP) and in thymic progenitors (ETP and DN2). |
| Breeding history | The original chimera was first crossed to C57BL/6J for germline transmission and a second generation. The colony was subsequently split into two branches: one branch was continuously backcrossed to C57BL/6J to generate the B6-Cpa3tm3(icre)Hrr line (EMMA strain EM:16832), whereas the other branch was established by using an F2 mouse to initiate backcrossing to BALB/c and to generate the C-Cpa3tm3(icre)Hrr line (this strain, EM:16833). The strain has been backcrossed to BALB/c for more than 20 generations. The strain is maintained by HET × WT brother–sister matings. |
| References |
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| Homozygous fertile | yes |
| Homozygous viable | yes |
| Homozygous matings required | no |
| Immunocompromised | no |
Information from EMMA
| Archiving centre | Helmholtz Zentrum Muenchen - German Research Center for Environmental Health (GmbH), Oberschleißheim, Germany |
| Animals used for archiving | heterozygous BALB/c males |
Disease and phenotype information
Literature references
- Cre-mediated cell ablation contests mast cell contribution in models of antibody- and T cell-mediated autoimmunity.;Feyerabend Thorsten B, Weiser Anne, Tietz Annette, Stassen Michael, Harris Nicola, Kopf Manfred, Radermacher Peter, Möller Peter, Benoist Christophe, Mathis Diane, Fehling Hans Jörg, Rodewald Hans-Reimer, ;2011;Immunity;35;832-44; 22101159
- Widespread immunological functions of mast cells: fact or fiction?;Rodewald Hans-Reimer, Feyerabend Thorsten B, ;2012;Immunity;37;13-24; 22840840
- Mast cells have no impact on cutaneous leishmaniasis severity and related Th2 differentiation in resistant and susceptible mice.;Paul Christoph, Wolff Svenja, Zapf Thea, Raifer Hartmann, Feyerabend Thorsten B, Bollig Nadine, Camara Bärbel, Trier Claudia, Schleicher Ulrike, Rodewald Hans-Reimer, Lohoff Michael, ;2016;European journal of immunology;46;114-21; 26449668
- Mucosal mast cells are indispensable for the timely termination of Strongyloides ratti infection.;Reitz M, Brunn M-L, Rodewald H-R, Feyerabend T B, Roers A, Dudeck A, Voehringer D, Jönsson F, Kühl A A, Breloer M, ;2017;Mucosal immunology;10;481-492; 27381924
- Foxp3⁺ regulatory T cells delay expulsion of intestinal nematodes by suppression of IL-9-driven mast cell activation in BALB/c but not in C57BL/6 mice.;Blankenhaus Birte, Reitz Martina, Brenz Yannick, Eschbach Marie-Luise, Hartmann Wiebke, Haben Irma, Sparwasser Tim, Huehn Jochen, Kühl Anja, Feyerabend Thorsten B, Rodewald Hans-Reimer, Breloer Minka, ;2014;PLoS pathogens;10;e1003913; 24516385
- Local immune response to food antigens drives meal-induced abdominal pain.;Aguilera-Lizarraga Javier, Florens Morgane V, Viola Maria Francesca, Jain Piyush, Decraecker Lisse, Appeltans Iris, Cuende-Estevez Maria, Fabre Naomi, Van Beek Kim, Perna Eluisa, Balemans Dafne, Stakenborg Nathalie, Theofanous Stavroula, Bosmans Goele, Mondelaers Stéphanie U, Matteoli Gianluca, Ibiza Martínez Sales, Lopez-Lopez Cintya, Jaramillo-Polanco Josue, Talavera Karel, Alpizar Yeranddy A, Feyerabend Thorsten B, Rodewald Hans-Reimer, Farre Ricard, Redegeld Frank A, Si Jiyeon, Raes Jeroen, Breynaert Christine, Schrijvers Rik, Bosteels Cédric, Lambrecht Bart N, Boyd Scott D, Hoh Ramona A, Cabooter Deirdre, Nelis Maxim, Augustijns Patrick, Hendrix Sven, Strid Jessica, Bisschops Raf, Reed David E, Vanner Stephen J, Denadai-Souza Alexandre, Wouters Mira M, Boeckxstaens Guy E, ;2021;Nature;590;151-156; 33442055
- Mast cell-derived serotonin enhances methacholine-induced airway hyperresponsiveness in house dust mite-induced experimental asthma.;Mendez-Enriquez Erika, Alvarado-Vazquez Perla Abigail, Abma Willem, Simonson Oscar E, Rodin Sergey, Feyerabend Thorsten B, Rodewald Hans-Reimer, Malinovschi Andrei, Janson Christer, Adner Mikael, Hallgren Jenny, ;2021;Allergy;76;2057-2069; 33486786