B6.Il7rtm1.1(icre)Hrr

Status	Under development - register interest
EMMA ID	EM:16834
Citation information	RRID:IMSR_EM:16834 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information.
International strain name	B6.Il7rtm1.1(icre)Hrr
Alternative name	B6.Il7rtm1.1(icre)Hrr
Strain type	Targeted Mutant Strains : Knock-in
Allele/Transgene symbol	Il7r^{tm1.1(icre)Hrr}
Gene/Transgene symbol	Il7r

Information from provider

Provider	Hans-Reimer Rodewald
Provider affiliation	Cellular Immunology D110, German Cancer Research Center
Genetic information	Codon-improved cre recombinase (icre) was inserted by homologous recombination in ES cells into the first exon of the Il7r gene. The targeting construct consisted, from 5' to 3', of a homology arm with sequence upstream of the Il7r ATG start site, a splice donor site, an intron and a splice acceptor site from the rabbit b-globin gene, the coding sequence of codon-improved cre (icre), a poly-adenylation signal from the rabbit b-globin gene, an FRT-flanked neomycin resistance gene, a 3' homology sequence and a diphtheria toxin subunit A negative selection sequence. Homologous recombination was carried out in E14.1 ES cells. To avoid potential impact of the neo resistance gene with its enhancer and promoter sequences on icre expression, the neo cassette was removed from the targeted locus by transient expression of flp recombinase, leaving only a single FRT site in the genome (Schlenner SM et al., 2010).
Phenotypic information	Homozygous: Mice are viable and fertile. Homozygous mice have reduced T and B cells and innate lymphoid lineages. Heterozygous: Mice are viable, fertile and immune-competent; cre recombinase is expressed in the T- and B-cell lineage.
Breeding history	Mice were backcrossed to C57BL/6J for more than 20 generations. Currently, the strain is maintained by HET × HOM brother–sister matings.
References	Light-entrained and brain-tuned circadian circuits regulate ILC3s and gut homeostasis.;Godinho-Silva Cristina, Domingues Rita G, Rendas Miguel, Raposo Bruno, Ribeiro Hélder, da Silva Joaquim Alves, Vieira Ana, Costa Rui M, Barbosa-Morais Nuno L, Carvalho Tânia, Veiga-Fernandes Henrique, ;2019;Nature;574;254-258; 31534216 The transcription factor RORα is required for the development of type 1 innate lymphoid cells in adult bone marrow.;Abe Shinya, Kagao Moe, Asahi Takuma, Kato Ryoma, Tani-Ichi Shizue, Shimba Akihiro, Ishibashi Riki, Miyachi Hitoshi, Kitano Satsuki, Miyazaki Masaki, Rodewald Hans-Reimer, Toyoshima Fumiko, Ikuta Koichi, ;2025;Journal of immunology (Baltimore, Md. : 1950);214;575-581; 40079843 Fate mapping reveals separate origins of T cells and myeloid lineages in the thymus.;Schlenner Susan M, Madan Vikas, Busch Katrin, Tietz Annette, Läufle Carolin, Costa Celine, Blum Carmen, Fehling Hans Jörg, Rodewald Hans-Reimer, ;2010;Immunity;32;426-36; 20303297 Bcl11b is essential for group 2 innate lymphoid cell development.;Walker Jennifer A, Oliphant Christopher J, Englezakis Alexandros, Yu Yong, Clare Simon, Rodewald Hans-Reimer, Belz Gabrielle, Liu Pentao, Fallon Padraic G, McKenzie Andrew N J, ;2015;The Journal of experimental medicine;212;875-82; 25964370 Group 2 innate lymphoid cells license dendritic cells to potentiate memory TH2 cell responses.;Halim Timotheus Y F, Hwang You Yi, Scanlon Seth T, Zaghouani Habib, Garbi Natalio, Fallon Padraic G, McKenzie Andrew N J, ;2016;Nature immunology;17;57-64; 26523868 Arginase 1 is an innate lymphoid-cell-intrinsic metabolic checkpoint controlling type 2 inflammation.;Monticelli Laurel A, Buck Michael D, Flamar Anne-Laure, Saenz Steven A, Tait Wojno Elia D, Yudanin Naomi A, Osborne Lisa C, Hepworth Matthew R, Tran Sara V, Rodewald Hans-Reimer, Shah Hardik, Cross Justin R, Diamond Joshua M, Cantu Edward, Christie Jason D, Pearce Erika L, Artis David, ;2016;Nature immunology;17;656-65; 27043409 Type-2 innate lymphoid cells control the development of atherosclerosis in mice.;Newland Stephen A, Mohanta Sarajo, Clément Marc, Taleb Soraya, Walker Jennifer A, Nus Meritxell, Sage Andrew P, Yin Changjun, Hu Desheng, Kitt Lauren L, Finigan Alison J, Rodewald Hans-Reimer, Binder Christoph J, McKenzie Andrew N J, Habenicht Andreas J, Mallat Ziad, ;2017;Nature communications;8;15781; 28589929 The E-Id Protein Axis Specifies Adaptive Lymphoid Cell Identity and Suppresses Thymic Innate Lymphoid Cell Development.;Miyazaki Masaki, Miyazaki Kazuko, Chen Kenian, Jin Yi, Turner Jacob, Moore Amanda J, Saito Rintaro, Yoshida Kenichi, Ogawa Seishi, Rodewald Hans-Reimer, Lin Yin C, Kawamoto Hiroshi, Murre Cornelis, ;2017;Immunity;46;818-834.e4; 28514688 β₂-adrenergic receptor-mediated negative regulation of group 2 innate lymphoid cell responses.;Moriyama Saya, Brestoff Jonathan R, Flamar Anne-Laure, Moeller Jesper B, Klose Christoph S N, Rankin Lucille C, Yudanin Naomi A, Monticelli Laurel A, Putzel Gregory Garbès, Rodewald Hans-Reimer, Artis David, ;2018;Science (New York, N.Y.);359;1056-1061; 29496881 The transcription factor c-Maf is essential for the commitment of IL-17-producing γδ T cells.;Zuberbuehler Matthew K, Parker Morgan E, Wheaton Joshua D, Espinosa Jaclyn R, Salzler Harmony R, Park Eunchong, Ciofani Maria, ;2019;Nature immunology;20;73-85; 30538336 Interleukin-33 Induces the Enzyme Tryptophan Hydroxylase 1 to Promote Inflammatory Group 2 Innate Lymphoid Cell-Mediated Immunity.;Flamar Anne-Laure, Klose Christoph S N, Moeller Jesper B, Mahlakõiv Tanel, Bessman Nicholas J, Zhang Wen, Moriyama Saya, Stokic-Trtica Vladislava, Rankin Lucille C, Putzel Gregory Garbès, Rodewald Hans-Reimer, He Zhengxiang, Chen Lili, Lira Sergio A, Karsenty Gerard, Artis David, ;2020;Immunity;52;606-619.e6; 32160524 Cross-talk between ILC2 and Gata3^high T_regs locally constrains adaptive type 2 immunity.;Stockis Julie, Yip Thomas, Moreno-Vicente Julia, Burton Oliver, Samarakoon Youhani, Schuijs Martijn J, Raghunathan Shwetha, Garcia Celine, Luo Weike, Whiteside Sarah K, Png Shaun, Simpson Charlotte, Monk Stela, Sawle Ashley, Yin Kelvin, Barbieri Johanna, Papadopoulos Panagiotis, Wong Hannah, Rodewald Hans-Reimer, Vyse Timothy, McKenzie Andrew N J, Cragg Mark S, Hoare Matthew, Withers David R, Fehling Hans Jörg, Roychoudhuri Rahul, Liston Adrian, Halim Timotheus Y F, ;2024;Science immunology;9;eadl1903; 39028828 ILC2 instructs neural stem and progenitor cells to potentiate neurorepair after stroke.;Liu Gaoyu, Huang Huachen, Wang Ying, Han Yali, Wang Jianye, Shi Mengxuan, Zhou Pan, Chen Chun, Yu Ying, Liu Qiang, Zhou Jie, ;2025;Neuron;113;1741-1757.e7; 40233748 The transcription factor NFIL3 drives innate lymphoid cell specification from lymphoid progenitors.;Léger Joseph, Artano Eva, Coulais Delphine, Belletoise Naomie, Fadhloun Raoudha, Kenney Devin, Bhandoola Avinash, Harly Christelle, ;2025;Immunity;58;2685-2703.e6; 41172988
Homozygous fertile	yes
Homozygous viable	yes
Homozygous matings required	no
Immunocompromised	no

Information from EMMA

Archiving centre	Helmholtz Zentrum Muenchen - German Research Center for Environmental Health (GmbH), Oberschleißheim, Germany
Animals used for archiving	heterozygous C57BL/6J males

Disease and phenotype information

Orphanet associated rare diseases, based on orthologous gene matching

Omenn syndrome / Orphanet_39041
T-B+ severe combined immunodeficiency due to IL-7Ralpha deficiency / Orphanet_169154

Literature references

Light-entrained and brain-tuned circadian circuits regulate ILC3s and gut homeostasis.;Godinho-Silva Cristina, Domingues Rita G, Rendas Miguel, Raposo Bruno, Ribeiro Hélder, da Silva Joaquim Alves, Vieira Ana, Costa Rui M, Barbosa-Morais Nuno L, Carvalho Tânia, Veiga-Fernandes Henrique, ;2019;Nature;574;254-258; 31534216
The transcription factor RORα is required for the development of type 1 innate lymphoid cells in adult bone marrow.;Abe Shinya, Kagao Moe, Asahi Takuma, Kato Ryoma, Tani-Ichi Shizue, Shimba Akihiro, Ishibashi Riki, Miyachi Hitoshi, Kitano Satsuki, Miyazaki Masaki, Rodewald Hans-Reimer, Toyoshima Fumiko, Ikuta Koichi, ;2025;Journal of immunology (Baltimore, Md. : 1950);214;575-581; 40079843
Fate mapping reveals separate origins of T cells and myeloid lineages in the thymus.;Schlenner Susan M, Madan Vikas, Busch Katrin, Tietz Annette, Läufle Carolin, Costa Celine, Blum Carmen, Fehling Hans Jörg, Rodewald Hans-Reimer, ;2010;Immunity;32;426-36; 20303297
Bcl11b is essential for group 2 innate lymphoid cell development.;Walker Jennifer A, Oliphant Christopher J, Englezakis Alexandros, Yu Yong, Clare Simon, Rodewald Hans-Reimer, Belz Gabrielle, Liu Pentao, Fallon Padraic G, McKenzie Andrew N J, ;2015;The Journal of experimental medicine;212;875-82; 25964370
Group 2 innate lymphoid cells license dendritic cells to potentiate memory TH2 cell responses.;Halim Timotheus Y F, Hwang You Yi, Scanlon Seth T, Zaghouani Habib, Garbi Natalio, Fallon Padraic G, McKenzie Andrew N J, ;2016;Nature immunology;17;57-64; 26523868
Arginase 1 is an innate lymphoid-cell-intrinsic metabolic checkpoint controlling type 2 inflammation.;Monticelli Laurel A, Buck Michael D, Flamar Anne-Laure, Saenz Steven A, Tait Wojno Elia D, Yudanin Naomi A, Osborne Lisa C, Hepworth Matthew R, Tran Sara V, Rodewald Hans-Reimer, Shah Hardik, Cross Justin R, Diamond Joshua M, Cantu Edward, Christie Jason D, Pearce Erika L, Artis David, ;2016;Nature immunology;17;656-65; 27043409
Type-2 innate lymphoid cells control the development of atherosclerosis in mice.;Newland Stephen A, Mohanta Sarajo, Clément Marc, Taleb Soraya, Walker Jennifer A, Nus Meritxell, Sage Andrew P, Yin Changjun, Hu Desheng, Kitt Lauren L, Finigan Alison J, Rodewald Hans-Reimer, Binder Christoph J, McKenzie Andrew N J, Habenicht Andreas J, Mallat Ziad, ;2017;Nature communications;8;15781; 28589929
The E-Id Protein Axis Specifies Adaptive Lymphoid Cell Identity and Suppresses Thymic Innate Lymphoid Cell Development.;Miyazaki Masaki, Miyazaki Kazuko, Chen Kenian, Jin Yi, Turner Jacob, Moore Amanda J, Saito Rintaro, Yoshida Kenichi, Ogawa Seishi, Rodewald Hans-Reimer, Lin Yin C, Kawamoto Hiroshi, Murre Cornelis, ;2017;Immunity;46;818-834.e4; 28514688
β₂-adrenergic receptor-mediated negative regulation of group 2 innate lymphoid cell responses.;Moriyama Saya, Brestoff Jonathan R, Flamar Anne-Laure, Moeller Jesper B, Klose Christoph S N, Rankin Lucille C, Yudanin Naomi A, Monticelli Laurel A, Putzel Gregory Garbès, Rodewald Hans-Reimer, Artis David, ;2018;Science (New York, N.Y.);359;1056-1061; 29496881
The transcription factor c-Maf is essential for the commitment of IL-17-producing γδ T cells.;Zuberbuehler Matthew K, Parker Morgan E, Wheaton Joshua D, Espinosa Jaclyn R, Salzler Harmony R, Park Eunchong, Ciofani Maria, ;2019;Nature immunology;20;73-85; 30538336
Interleukin-33 Induces the Enzyme Tryptophan Hydroxylase 1 to Promote Inflammatory Group 2 Innate Lymphoid Cell-Mediated Immunity.;Flamar Anne-Laure, Klose Christoph S N, Moeller Jesper B, Mahlakõiv Tanel, Bessman Nicholas J, Zhang Wen, Moriyama Saya, Stokic-Trtica Vladislava, Rankin Lucille C, Putzel Gregory Garbès, Rodewald Hans-Reimer, He Zhengxiang, Chen Lili, Lira Sergio A, Karsenty Gerard, Artis David, ;2020;Immunity;52;606-619.e6; 32160524
Cross-talk between ILC2 and Gata3^high T_regs locally constrains adaptive type 2 immunity.;Stockis Julie, Yip Thomas, Moreno-Vicente Julia, Burton Oliver, Samarakoon Youhani, Schuijs Martijn J, Raghunathan Shwetha, Garcia Celine, Luo Weike, Whiteside Sarah K, Png Shaun, Simpson Charlotte, Monk Stela, Sawle Ashley, Yin Kelvin, Barbieri Johanna, Papadopoulos Panagiotis, Wong Hannah, Rodewald Hans-Reimer, Vyse Timothy, McKenzie Andrew N J, Cragg Mark S, Hoare Matthew, Withers David R, Fehling Hans Jörg, Roychoudhuri Rahul, Liston Adrian, Halim Timotheus Y F, ;2024;Science immunology;9;eadl1903; 39028828
ILC2 instructs neural stem and progenitor cells to potentiate neurorepair after stroke.;Liu Gaoyu, Huang Huachen, Wang Ying, Han Yali, Wang Jianye, Shi Mengxuan, Zhou Pan, Chen Chun, Yu Ying, Liu Qiang, Zhou Jie, ;2025;Neuron;113;1741-1757.e7; 40233748
The transcription factor NFIL3 drives innate lymphoid cell specification from lymphoid progenitors.;Léger Joseph, Artano Eva, Coulais Delphine, Belletoise Naomie, Fadhloun Raoudha, Kenney Devin, Bhandoola Avinash, Harly Christelle, ;2025;Immunity;58;2685-2703.e6; 41172988

Information on how we integrate external resources can be found here

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

Frozen sperm. Delivered in 4 weeks (after paperwork in place). €1740^*

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

^* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
Distribution of this strain is subject to a provider MTA. Both signing of the MTA and submission of the online EMMA Mutant Request Form are required before material can be shipped.

EMMA conditions
Legally binding conditions for the transfer

Right strain for your research?

The information provided on this page is, to the best of EMMA’s knowledge, based on data supplied by the original provider. End users are responsible for reviewing these details and for validating the strain and its suitability for their experimental use.
Not found what you were looking for? Search here for other strains available from EMMA.