IMPC phenotypes (gene matching)
Tektm1.1(icre/Esr1*)Hrr (Tie2-MicM)
| Status | Under development - register interest |
| EMMA ID | EM:16839 |
| Citation information | RRID:IMSR_EM:16839 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information. |
| International strain name | Tektm1.1(icre/Esr1*)Hrr (Tie2-MicM) |
| Alternative name | Tektm1.1(icre/Esr1*)Hrr (Tie2-MicM) |
| Strain type | Targeted Mutant Strains : Knock-in |
| Allele/Transgene symbol | Tektm1.1(icre/Esr1*)Hrr |
| Gene/Transgene symbol | Tek |
Information from provider
| Provider | Hans-Reimer Rodewald |
| Provider affiliation | Cellular Immunology D110, German Cancer Research Center |
| Genetic information | The tamoxifen-inducible Mer-iCre-Mer was introduced into the first exon of the Tie2 gene by homologous recombination in ES cells. The targeting cassette inserted into exon 1, upstream of the translation start site, comprises a rabbit HBB2 splice donor, intron, and splice acceptor; an inducible improved cre recombinase fused to two modified Esr1 binding domains (icre/Esr1*); and an FRT-flanked neomycin resistance cassette; flp-mediated recombination removed the selection cassette (Busch K. et al., Nature, 2015, PubMed ID:25686605). |
| Phenotypic information | Homozygous:Homozygous mice are embryonic lethal between E9.5 and E12.5.Heterozygous:Heterozygous mice are viable and fertile and show no apparent abnormalities. Heterozygous mice are not immunodeficient and have normal numbers of immune and blood cells. |
| Breeding history | Mice were backcrossed to C57BL/6J for more than 25 generations. |
| References |
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| Homozygous fertile | no |
| Homozygous viable | no |
| Homozygous matings required | no |
| Immunocompromised | no |
Information from EMMA
| Archiving centre | Helmholtz Zentrum Muenchen - German Research Center for Environmental Health (GmbH), Oberschleißheim, Germany |
| Animals used for archiving | heterozygous C57BL/6J males |
Disease and phenotype information
Literature references
- Tissue-resident macrophages originate from yolk-sac-derived erythro-myeloid progenitors.;Gomez Perdiguero Elisa, Klapproth Kay, Schulz Christian, Busch Katrin, Azzoni Emanuele, Crozet Lucile, Garner Hannah, Trouillet Celine, de Bruijn Marella F, Geissmann Frederic, Rodewald Hans-Reimer, ;2015;Nature;518;547-51; 25470051
- Fundamental properties of unperturbed haematopoiesis from stem cells in vivo.;Busch Katrin, Klapproth Kay, Barile Melania, Flossdorf Michael, Holland-Letz Tim, Schlenner Susan M, Reth Michael, Höfer Thomas, Rodewald Hans-Reimer, ;2015;Nature;518;542-6; 25686605
- Differentiation-based model of hematopoietic stem cell functions and lineage pathways.;Höfer Thomas, Rodewald Hans-Reimer, ;2018;Blood;132;1106-1113; 30042097
- Resolving Fates and Single-Cell Transcriptomes of Hematopoietic Stem Cell Clones by PolyloxExpress Barcoding.;Pei Weike, Shang Fuwei, Wang Xi, Fanti Ann-Kathrin, Greco Alessandro, Busch Katrin, Klapproth Kay, Zhang Qin, Quedenau Claudia, Sauer Sascha, Feyerabend Thorsten B, Höfer Thomas, Rodewald Hans-Reimer, ;2020;Cell stem cell;27;383-395.e8; 32783885
- Usp22 is an intracellular regulator of systemic emergency hematopoiesis.;Dietlein Nikolaus, Wang Xi, Metz Jonas, Disson Olivier, Shang Fuwei, Beyersdörffer Celine, Rodríguez Correa Esther, Lipka Daniel B, Begus-Nahrmann Yvonne, Kosinsky Robyn Laura, Johnsen Steven A, Lecuit Marc, Höfer Thomas, Rodewald Hans-Reimer, ;2022;Science immunology;7;eabq2061; 36490327
- Flt3- and Tie2-Cre tracing identifies regeneration in sepsis from multipotent progenitors but not hematopoietic stem cells.;Fanti Ann-Kathrin, Busch Katrin, Greco Alessandro, Wang Xi, Cirovic Branko, Shang Fuwei, Nizharadze Tamar, Frank Larissa, Barile Melania, Feyerabend Thorsten B, Höfer Thomas, Rodewald Hans-Reimer, ;2023;Cell stem cell;30;207-218.e7; 36652946
- Differentiation tracing identifies hematopoietic regeneration from multipotent progenitors but not stem cells.;Nizharadze Tamar, Busch Katrin, Fanti Ann-Kathrin, Rodewald Hans-Reimer, Höfer Thomas, ;2023;Cells & development;175;203861; 37286105
- Vasopressin drives aberrant myeloid differentiation of hematopoietic stem cells, contributing to depression in mice.;Mou Rong, Ma Junkai, Ju Xuan, Wu Yixin, Chen Qiuli, Li Jinglin, Shang Tongyao, Chen Siying, Yang Yue, Li Yue, Lv Kaosheng, Chen Xuequn, Zhang Qi, Liang Tingbo, Feng Ye, Lu Xinjiang, ;2024;Cell stem cell;31;1794-1812.e10; 39442524
- Multipotent progenitors with distinct origins, clonal lineage fates, transcriptomes, and surface markers yield two hematopoietic trees.;Shang Fuwei, Nizharadze Tamar, Thiele Robin, Cirovic Branko, Frank Larissa, Busch Katrin, Pei Weike, Feyerabend Thorsten B, Höfer Thomas, Wang Xi, Rodewald Hans-Reimer, ;2025;Proceedings of the National Academy of Sciences of the United States of America;122;e2505510122; 41284889