IMPC phenotypes (gene matching)
C57BL/6J-Trp73em1Fcau/Orl
| Status | Under development - register interest |
| EMMA ID | EM:17006 |
| Citation information | RRID:IMSR_EM:17006 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information. |
| International strain name | C57BL/6J-Trp73em1Fcau/Orl |
| Alternative name | Trp73tm1Fcau (TAp73flox, Trp73floxE2-3) |
| Strain type | Endonuclease-mediated |
| Allele/Transgene symbol | Trp73em1Fcau |
| Gene/Transgene symbol | Trp73 |
Information from provider
| Provider | Frédéric CAUSERET |
| Provider affiliation | Imagine Institute, Inserm |
| Genetic information | LoxP sites were inserted upstream of exon 2 and downstream of exon 3, which are specific to the transcriptionally active isoforms of p73 (TAp73). Cre-mediated recombination results in the complete loss of TAp73 immunoreactivity. CRISPR/Cas9 details: The line was generated by electroporation of CRISPR/Cas9 ribonucleoprotein (RNP) complexes into C57BL/6J mouse zygotes. Cas9 protein, sgRNA, and the ssODN repair template were purchased from Integrated DNA Technologies (IDT). |
| Phenotypic information | Homozygous:Homozygous floxed (loxP/loxP) mice are viable and fertile. The viability, fertility and behavior of homozygous knockout (KO) mice following Cre-mediated recombination have not yet been assessed.Heterozygous:Heterozygous lox/+ mice are viable and fertile. Following Cre-mediated recombination, heterozygous animals are viable and fertile, with no obvious phenotype detected. |
| Breeding history | The strain was generated by electroporation of CRISPR/Cas9 ribonucleoprotein (RNP) complexes into C57BL/6J mouse zygotes. Founder animals were validated by PCR and backcrossed to C57BL/6J mice. |
| References | None available |
| Homozygous fertile | yes |
| Homozygous viable | yes |
| Homozygous matings required | yes |
| Immunocompromised | no |
Information from EMMA
| Archiving centre | CNRS-TAAM – Typing and Archiving of Animal Models, Orléans, France |
| Animals used for archiving | homozygous C57BL/6J males |
Disease and phenotype information
MGI phenotypes (gene matching)
- absent Cajal-Retzius cell / MGI
- domed cranium / MGI
- gastrointestinal hemorrhage / MGI
- absent enterocytes / MGI
- excessive digestive secretion / MGI
- abnormal cerebral cortex morphology / MGI
- abnormal cortical marginal zone morphology / MGI
- abnormal hippocampus morphology / MGI
- abnormal hippocampus development / MGI
- abnormal dentate gyrus morphology / MGI
- abnormal choroid plexus morphology / MGI
- dilated lateral ventricles / MGI
- abnormal hypothalamus morphology / MGI
- abnormal trigeminal ganglion morphology / MGI
- decreased body size / MGI
- submission towards male mice / MGI
- reduced male mating frequency / MGI
- abnormal embryo development / MGI
- periorbital edema / MGI
- increased susceptibility to otitis media / MGI
- conjunctivitis / MGI
- rhinitis / MGI
- sinus inflammation / MGI
- hydroencephaly / MGI
- non-obstructive hydrocephaly / MGI
- abnormal cerebrospinal fluid production / MGI
- intracranial hemorrhage / MGI
- intraventricular hemorrhage / MGI
- reduced fertility / MGI
- male infertility / MGI
- female infertility / MGI
- abnormal ovulation / MGI
- neoplasm / MGI
- increased cellular sensitivity to gamma-irradiation / MGI
- increased malignant tumor incidence / MGI
- increased tumor incidence / MGI
- increased T cell derived lymphoma incidence / MGI
- increased lung adenocarcinoma incidence / MGI
- increased carcinoma incidence / MGI
- increased lung adenoma incidence / MGI
- premature death / MGI
- neurodegeneration / MGI
- abnormal vomeronasal organ morphology / MGI
- increased susceptibility to bacterial infection / MGI
- chronic inflammation / MGI
- abnormal hippocampal mossy fiber morphology / MGI
- abnormal cell cycle / MGI
- increased neuron apoptosis / MGI
- abnormal forebrain development / MGI
- increased hemangiosarcoma incidence / MGI
- maternal effect / MGI
- abnormal chromosome number / MGI
- polyploidy / MGI
- increased incidence of tumors by chemical induction / MGI
- cachexia / MGI
- abnormal female meiosis / MGI
- decreased oocyte number / MGI
- decreased Cajal-Retzius cell number / MGI
- abnormal dendrite morphology / MGI
- abnormal hippocampus CA3 region morphology / MGI
- anovulation / MGI
- abnormal ovarian follicle number / MGI
- increased physiological sensitivity to xenobiotic / MGI
- increased sensitivity to induced cell death / MGI
- decreased neuron number / MGI
- distended ileum / MGI
- increased thymocyte apoptosis / MGI
- abnormal meiotic spindle morphology / MGI
- abnormal hippocampus neuron morphology / MGI
- postnatal lethality, incomplete penetrance / MGI
- prenatal lethality, incomplete penetrance / MGI
- increased lymphoma incidence / MGI