MGI phenotypes (allele matching)
B6.129-Rasd2tm1Rdl/Cnrm
| Status | Available to order |
| EMMA ID | EM:01823 |
| International strain name | B6.129-Rasd2tm1Rdl/Cnrm |
| Alternative name | RHES KO |
| Strain type | Targeted Mutant Strains : Knock-out |
| Allele/Transgene symbol | Rasd2tm1Rdl, |
| Gene/Transgene symbol | Rasd2 |
Information from provider
| Provider | Roberto DI LAURO |
| Provider affiliation | BioGeM |
| Genetic information | The Rasd2 (or Rhes, Ras homolog enriched in striatum) gene encodes for a 266-aminoacid protein, predominantly expressed in the striatum, that belongs to the RASD subfamily of the Ras-related GTP-binding protein superfamily. We generated mice carrying null mutations in the Rhes gene by gene-targeting techniques. The Rhes genomic locus was isolated by genotyping screening of the phage artificial chromosome library RPCI-21 (provided by the Yac Screening Center, DIBIT-HSR and IGBE/IGM-CNR, Milan, Italy). The internal ribosome entry site-EGFP cassette and the PGKneo cassette, flanked by loxP sequences, were flanked by two Rhes genomic DNA fragments: a 2.9 kb fragment including the Rhes translational start codon and a 5 kb fragment. The 2.9 kb genomic fragment underwent a site-directed mutagenesis reaction which allowed insertion of a stop codon in each reading frame and EcoRI and XhoI restriction sites downstream of the Rhes translational start codon. |
| Phenotypic information | Mice homozygous for the Rasd2 (Rhes) mutation are viable and fertile but smaller than wild-type mice. Furthermore, they show a gender-dependent increase in anxiety levels and a motor coordination deficit but no learning or memory impairment. |
| References |
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Information from EMMA
| Archiving centre | CNR, Consiglio Nazionale delle Ricerche, Monterotondo, Italy |
Disease and phenotype information
Literature references
- Rhes is involved in striatal function.;Spano Daniela, Branchi Igor, Rosica Annamaria, Pirro Maria Teresa, Riccio Antonio, Mithbaokar Pratibha, Affuso Andrea, Arra Claudio, Campolongo Patrizia, Terracciano Daniela, Macchia Vincenzo, Bernal Juan, Alleva Enrico, Di Lauro Roberto, ;2004;Molecular and cellular biology;24;5788-96; 15199135