MGI phenotypes (allele matching)
- no phenotypic analysis / MGI
B6;129P2-Gjd3tm1.1Kwi/Cnrm
| Status | Available to order |
| EMMA ID | EM:01915 |
| International strain name | B6;129P2-Gjd3tm1.1Kwi/Cnrm |
| Alternative name | Cx30.2 flox |
| Strain type | Targeted Mutant Strains : Conditional mutation |
| Allele/Transgene symbol | Gjd3tm1.1Kwi, |
| Gene/Transgene symbol | Gjd3 |
Information from provider
| Provider | Klaus WILLECKE |
| Provider affiliation | Molekulargenetik, Institut fuer Genetik, Universitaet Bonn |
| Genetic information | Instead of the whole open reading frame of exon 2 of Cx30.2 (Gjd3, formerly Gjc1) a loxP sites-flanked construct is inserted, containing the exon 2 of Cx30.2 and, 3' of the poly(A)-signal), one frt sites-flanked pgk-neomycin selection cassette. After the second loxP site a lacZ reporter gene cassette is inserted. After cre recombinase-mediated deletion of Cx30.2, lacZ is expressed under the control of the endogenous Cx30.2 promoter. |
| Phenotypic information | None in the loxP-flanked state. After cre recombinase-mediated deletion homozygous lacZ/lacZ mice are nearly normal. |
| Breeding history | After blastocyst injection the chimeras were bred with C57BL/6NCrl to obtain brown coat colored offspring. This brown offspring was backcrossed to C57BL/6NCrl more than 3 times. |
| References |
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Information from EMMA
| Archiving centre | CNR, Consiglio Nazionale delle Ricerche, Monterotondo, Italy |
| Breeding at archiving centre | Backcrossed to C57BL/6J |
Disease and phenotype information
Literature references
- Connexin30.2 containing gap junction channels decelerate impulse propagation through the atrioventricular node.;Kreuzberg Maria M, Schrickel Jan W, Ghanem Alexander, Kim Jung-Sun, Degen Joachim, Janssen-Bienhold Ulrike, Lewalter Thorsten, Tiemann Klaus, Willecke Klaus, ;2006;Proceedings of the National Academy of Sciences of the United States of America;103;5959-64; 16571663