C57BL/6JSfdAnu-Ikzf1Plstc/ApbH

Status

Available to order

EMMA IDEM:02169
International strain nameC57BL/6JSfdAnu-Ikzf1Plstc/ApbH
Alternative namePlastic, C57BL/6Apb-Ikzf1/Apb
Strain typeInduced Mutant Strains : Chemically-induced
Allele/Transgene symbolIkzf1plstc,
Gene/Transgene symbolIkzf1

Information from provider

ProviderChris Goodnow
Provider affiliationThe Australian National University
Genetic informationThis stock carries A to G point mutation at nucleotide 572 (H191R) resulting in protein domain inactivation. A central issue in understanding the hematolymphoid system is the generation of appropriate mutant alleles in mice to reveal the function of regulatory genes. During systematically screening of the mouse genome for autoimmune regulators, the mouse strain, Plastic, was identified, with a point mutation in a zinc finger of Ikaros that disrupts DNA binding but preserves efficient assembly of the full-length protein into higher order complexes. Ikaros (Plastic) homozygosity is embryonically lethal with severe defects in terminal erythrocyte and granulocyte differentiation, excessive macrophage formation, and blocked lymphopoiesis, while heterozygotes display a partial block in lymphocyte differentiation. The contrast with more circumscribed effects of Ikaros alleles that ablate the full-length protein highlights the importance in mammals of generating recessive niche-filling alleles.
Phenotypic informationThis strain has the following phenotypic effects: Embryonic lethal E15.5-17.5. Anemia. Failure of normal erythroblast growth and differentiation in fetal liver Expansion of myeloid cells including: CD34+, CD45+, FcaR+ and Mac-1hi and Gr-1lo cell populations. No detectable Gr-1hi cells. Early lymphocyte development arrest. Accumulation of precursor B cells in thymus at expense of T cell development. Premature down regulation of c-kit expression. Cell intrinsic defect. Fetal liver cells fail to engraft.
References
  • Widespread failure of hematolymphoid differentiation caused by a recessive niche-filling allele of the Ikaros transcription factor.;Papathanasiou Peter, Perkins Andrew C, Cobb Bradley S, Ferrini Roger, Sridharan Rupa, Hoyne Gerard F, Nelms Keats A, Smale Stephen T, Goodnow Christopher C, ;2003;Immunity;19;131-44; 12871645

Information from EMMA

Archiving centreMary Lyon Centre at MRC Harwell, Oxford, United Kingdom

Disease and phenotype information

Orphanet associated rare diseases, based on orthologous gene matching

MGI phenotypes (allele matching)
  • liver hypoplasia / MGI
  • decreased thymocyte number / MGI
  • increased T cell derived lymphoma incidence / MGI
  • increased leukemia incidence / MGI
  • premature death / MGI
  • abnormal B cell differentiation / MGI
  • abnormal T cell differentiation / MGI
  • abnormal lymphopoiesis / MGI
  • decreased double-negative T cell number / MGI
  • decreased double-positive T cell number / MGI
  • increased pro-B cell number / MGI
  • decreased pre-B cell number / MGI
  • decreased mature B cell number / MGI
  • myeloid hyperplasia / MGI
  • decreased hematocrit / MGI
  • decreased granulocyte number / MGI
  • anemia / MGI
  • abnormal myelopoiesis / MGI
  • arrested B cell differentiation / MGI
  • thymus hypoplasia / MGI
  • decreased hemoglobin content / MGI
  • decreased erythrocyte cell number / MGI
  • pallor / MGI
  • increased macrophage cell number / MGI
  • absent T cells / MGI
  • abnormal granulocyte differentiation / MGI
  • abnormal macrophage differentiation / MGI
  • absent pro-B cells / MGI
  • reticulocytopenia / MGI
  • decreased erythroid progenitor cell number / MGI
  • lethality throughout fetal growth and development, complete penetrance / MGI
  • abnormal erythroblast morphology / MGI
  • decreased fetal derived definitive erythrocyte cell number / MGI
  • abnormal erythropoiesis / MGI

Literature references

  • Widespread failure of hematolymphoid differentiation caused by a recessive niche-filling allele of the Ikaros transcription factor.;Papathanasiou Peter, Perkins Andrew C, Cobb Bradley S, Ferrini Roger, Sridharan Rupa, Hoyne Gerard F, Nelms Keats A, Smale Stephen T, Goodnow Christopher C, ;2003;Immunity;19;131-44; 12871645

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Order

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

  • Frozen sperm. Delivered in 4 weeks (after paperwork in place). €1740*
  • Rederivation of mice from frozen stock, delivery time available upon request . €3880*

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

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