MGI phenotypes (allele matching)
B6;Cg-Gjb5tm1Kwi/Cnrm
| Status | Available to order |
| EMMA ID | EM:02382 |
| International strain name | B6;Cg-Gjb5tm1Kwi/Cnrm |
| Alternative name | Cx31.1LacZ |
| Strain type | Targeted Mutant Strains : Knock-out |
| Allele/Transgene symbol | Gjb5tm1Kwi, |
| Gene/Transgene symbol | Gjb5 |
Information from provider
| Provider | Klaus WILLECKE |
| Provider affiliation | Molekulargenetik, Institut fuer Genetik, Universitaet Bonn |
| Genetic information | Instead of the whole open reading frame of exon 2 of Cx31.1 (Gjb5), the coding region of the reporter gene LacZ is inserted. One loxP site, 3' of the poly(A)-signal, is remaining in the genome, upon deletion of the loxP-flanked HPRT-selection cassette. |
| Phenotypic information | Reduced knock-out survival. |
| Breeding history | After blastocyst injection the chimeras were bred with C57BL/6NCrl to obtain brown offspring. This brown offspring was backcrossed once with pgk-cre mice (nearly 100% C57BL/6NCrl) to delete the selection cassette and after that more than two times with C57BL/6NCrl. |
| References |
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Information from EMMA
| Archiving centre | CNR, Consiglio Nazionale delle Ricerche, Monterotondo, Italy |
| Breeding at archiving centre | Backcrossed to C57BL/6J |
Disease and phenotype information
Literature references
- Characterization of connexin31.1-deficient mice reveals impaired placental development.;Zheng-Fischhöfer Qingyi, Kibschull Mark, Schnichels Marc, Kretz Markus, Petrasch-Parwez Elisabeth, Strotmann Jörg, Reucher Harald, Lynn Bruce D, Nagy James I, Lye Stephen J, Winterhager Elke, Willecke Klaus, ;2007;Developmental biology;312;258-71; 17961533