MGI phenotypes (allele matching)
- impaired olfaction / MGI
B6.129P2(SJL)-Gjb4tm1Kwi/Cnrm
| Status | Available to order |
| EMMA ID | EM:02383 |
| International strain name | B6.129P2(SJL)-Gjb4tm1Kwi/Cnrm |
| Alternative name | Cx30.3LacZ |
| Strain type | Targeted Mutant Strains : Knock-out |
| Allele/Transgene symbol | Gjb4tm1Kwi, |
| Gene/Transgene symbol | Gjb4 |
Information from provider
| Provider | Klaus WILLECKE |
| Provider affiliation | Molekulargenetik, Institut fuer Genetik, Universitaet Bonn |
| Genetic information | Instead of the whole open reading frame of exon 2 of Gjb4 (Cx30.3), the coding region of the reporter gene LacZ is inserted. One frt site, 3' of the poly(A)-signal, is remaining in the genome, upon deletion of the frt-flanked neomycin selection cassette. |
| Phenotypic information | Reduced knock-out survival. |
| Breeding history | After blastocyst injection the chimeras were bred with C57BL/6NCrl to obtain brown offspring. This brown offspring was backcrossed once with flp recombinase deleter mice (nearly 100% C57BL/6NCrl) to delete the selection cassette and after that more than two times with C57BL/6NCrl. |
| References |
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Information from EMMA
| Archiving centre | CNR, Consiglio Nazionale delle Ricerche, Monterotondo, Italy |
| Breeding at archiving centre | Backcrossed to C57BL/6J |
Disease and phenotype information
Orphanet associated rare diseases, based on orthologous gene matching
- Erythrokeratodermia variabilis / Orphanet_317
Literature references
- Characterization of connexin30.3-deficient mice suggests a possible role of connexin30.3 in olfaction.;Zheng-Fischhöfer Qingyi, Schnichels Marc, Dere Ekrem, Strotmann Jörg, Loscher Nadine, McCulloch Fiona, Kretz Markus, Degen Joachim, Reucher Harald, Nagy James I, Peti-Peterdi Janos, Huston Joseph P, Breer Heinz, Willecke Klaus, ;2007;European journal of cell biology;86;683-700; 17728008