B6.129P2(SJL)-Gja1tm7(Gja2)Kwi/Cnrm

Status

Available to order

EMMA IDEM:02385
International strain nameB6.129P2(SJL)-Gja1tm7(Gja2)Kwi/Cnrm
Alternative nameCx43KICx26
Strain typeTargeted Mutant Strains : Knock-in
Allele/Transgene symbolGja1tm7(Gja2)Kwi,
Gene/Transgene symbolGja1

Information from provider

ProviderKlaus WILLECKE
Provider affiliationMolekulargenetik, Institut fuer Genetik, Universitaet Bonn
Genetic informationInstead of the whole open reading frame of exon 2 of Cx43 (Gja1) the coding region of Cx26 is inserted; one frt site, 3' of the poly(A)-signal, is remaining in the genome, upon deletion of the frt sites-flanked neomycin selection cassette.
Phenotypic informationHeterozygous females have lactation difficulties, homozygous mice are infertile.
Breeding historyAfter blastocyst injection the chimeras were bred with C57BL/6NCrl to obtain brown offspring. This brown offspring was backcrossed once with flp recombinase deleter mice (nearly 100% C57BL/6NCrl) to delete the selection cassette and after that more than three times with C57BL/6NCrl.
References
  • Replacement of connexin43 by connexin26 in transgenic mice leads to dysfunctional reproductive organs and slowed ventricular conduction in the heart.;Winterhager Elke, Pielensticker Nicole, Freyer Jennifer, Ghanem Alexander, Schrickel Jan W, Kim Jung-Sun, Behr Rüdiger, Grümmer Ruth, Maass Karen, Urschel Stephanie, Lewalter Thorsten, Tiemann Klaus, Simoni Manuela, Willecke Klaus, ;2007;BMC developmental biology;7;26; 17408477

Information from EMMA

Archiving centreCNR, Consiglio Nazionale delle Ricerche, Monterotondo, Italy
Breeding at archiving centreBackcrossed to C57BL/6J

Disease and phenotype information

Orphanet associated rare diseases, based on orthologous gene matching

MGI phenotypes (allele matching)
  • decreased heart rate / MGI
  • abnormal mammary gland growth during pregnancy / MGI
  • small ovary / MGI
  • abnormal ovarian follicle morphology / MGI
  • absent corpus luteum / MGI
  • abnormal spermatogenesis / MGI
  • decreased body weight / MGI
  • abnormal cardiovascular system physiology / MGI
  • postnatal growth retardation / MGI
  • male infertility / MGI
  • female infertility / MGI
  • ovary cysts / MGI
  • small heart / MGI
  • shortened QT interval / MGI
  • abnormal atrioventricular bundle conduction / MGI
  • decreased testis weight / MGI
  • azoospermia / MGI
  • abnormal spermatocyte morphology / MGI
  • prolonged QRS complex duration / MGI
  • postnatal lethality, incomplete penetrance / MGI
  • prenatal lethality, incomplete penetrance / MGI

Literature references

  • Replacement of connexin43 by connexin26 in transgenic mice leads to dysfunctional reproductive organs and slowed ventricular conduction in the heart.;Winterhager Elke, Pielensticker Nicole, Freyer Jennifer, Ghanem Alexander, Schrickel Jan W, Kim Jung-Sun, Behr Rüdiger, Grümmer Ruth, Maass Karen, Urschel Stephanie, Lewalter Thorsten, Tiemann Klaus, Simoni Manuela, Willecke Klaus, ;2007;BMC developmental biology;7;26; 17408477

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Order

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

  • Frozen embryos. Delivered in 4 weeks (after paperwork in place). €1740*
  • Rederivation of mice from frozen stock, delivery time available upon request . €3880*

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
For this strain no provider MTA is needed. Distribution is based on the EMMA conditions only.

EMMA conditions
Legally binding conditions for the transfer

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