B6.Cg-Ptprca Rag2tm1Fwa Foxp3tm1.1Mal Tg(TcraH-Y,TcrbH-Y)1Pas/Orl

Status

Available to order

EMMA IDEM:02419
International strain nameB6.Cg-Ptprca Rag2tm1Fwa Foxp3tm1.1Mal Tg(TcraH-Y,TcrbH-Y)1Pas/Orl
Alternative nameSimone
Strain typeTargeted Mutant Strains : Knock-out
Allele/Transgene symbolRag2tm1Fwa,
Gene/Transgene symbolRag2

Information from provider

ProviderDavid GROSS
Provider affiliationINSERM U580
Additional ownerO. Lantz (Marilyn) and B. Malissen (Foxp3 EGFP)
Genetic informationTCR transgenic T cells with "green" regulatory T cells (Tregs). The Foxp3 IRES-EGFP targeted knock-in allele (Foxp3 EGFP) was put on the Marilyn background. Transgenic for T(TCR)Valpha 1.1/Vbeta6 receptor specific of the H-Y male antigen presented by I-a b. The recognized peptide (NAGFNSNRANSSRSS) comes from Dby. As the clone originates from a F1 H-2b/k mouse, the TCR does not react with H-2k or H-2b. The mice of this strain are also homozygous for a Rag2 knock-out allele and Ptprca (CD45.1). An IRES-EGFP cassette was introduced in the 3'UTR region of FoxP3 gene, exon 11. The autodeleter neomycin-cre recombinase cassette was used.
Phenotypic informationNone.
References
  • Gamma chain required for naïve CD4+ T cell survival but not for antigen proliferation.;Lantz O, Grandjean I, Matzinger P, Di Santo J P, ;2000;Nature immunology;1;54-8; 10881175
  • Th2 lymphoproliferative disorder of LatY136F mutant mice unfolds independently of TCR-MHC engagement and is insensitive to the action of Foxp3+ regulatory T cells.;Wang Ying, Kissenpfennig Adrien, Mingueneau Michael, Richelme Sylvie, Perrin Pierre, Chevrier Stéphane, Genton Céline, Lucas Bruno, DiSanto James P, Acha-Orbea Hans, Malissen Bernard, Malissen Marie, ;2008;Journal of immunology (Baltimore, Md. : 1950);180;1565-75; 18209052

Information from EMMA

Archiving centreInstitut de Transgenose, INTRAGENE, Orléans, France

Disease and phenotype information

Orphanet associated rare diseases, based on orthologous gene matching

MGI phenotypes (allele matching)
  • increased granulocyte number / MGI
  • abnormal spleen morphology / MGI
  • spleen hypoplasia / MGI
  • decreased body size / MGI
  • arrested B cell differentiation / MGI
  • decreased IgM level / MGI
  • thymus hypoplasia / MGI
  • arrested T cell differentiation / MGI
  • increased susceptibility to infection / MGI
  • abnormal effector T cell morphology / MGI
  • increased natural killer cell mediated cytotoxicity / MGI
  • decreased double-positive T cell number / MGI
  • increased macrophage cell number / MGI
  • decreased CD4-positive, alpha beta T cell number / MGI
  • decreased CD8-positive, alpha-beta T cell number / MGI
  • lymph node hypoplasia / MGI
  • absent mature B cells / MGI
  • abnormal T cell receptor V(D)J recombination / MGI
  • abnormal immunoglobulin V(D)J recombination / MGI
  • decreased thymocyte number / MGI
  • lung inflammation / MGI
  • increased susceptibility to bacterial infection / MGI
  • abnormal macrophage physiology / MGI
  • colitis / MGI
  • abnormal T-helper 1 physiology / MGI
  • abnormal response to transplant / MGI
  • abnormal interferon secretion / MGI
  • abnormal chemokine secretion / MGI
  • decreased susceptibility to bacterial infection induced morbidity/mortality / MGI
  • abnormal intestinal mucosa morphology / MGI
  • abnormal B cell morphology / MGI
  • decreased susceptibility to parasitic infection / MGI
  • decreased double-negative T cell number / MGI
  • increased double-negative T cell number / MGI
  • decreased susceptibility to type IV hypersensitivity reaction / MGI
  • abnormal stomach mucosa morphology / MGI
  • abnormal intestinal epithelium morphology / MGI
  • abnormal B cell number / MGI
  • decreased pre-B cell number / MGI
  • decreased mature B cell number / MGI
  • increased immature B cell number / MGI
  • decreased immature B cell number / MGI
  • abnormal gamma-delta T cell differentiation / MGI

Literature references

  • Gamma chain required for naïve CD4+ T cell survival but not for antigen proliferation.;Lantz O, Grandjean I, Matzinger P, Di Santo J P, ;2000;Nature immunology;1;54-8; 10881175
  • Th2 lymphoproliferative disorder of LatY136F mutant mice unfolds independently of TCR-MHC engagement and is insensitive to the action of Foxp3+ regulatory T cells.;Wang Ying, Kissenpfennig Adrien, Mingueneau Michael, Richelme Sylvie, Perrin Pierre, Chevrier Stéphane, Genton Céline, Lucas Bruno, DiSanto James P, Acha-Orbea Hans, Malissen Bernard, Malissen Marie, ;2008;Journal of immunology (Baltimore, Md. : 1950);180;1565-75; 18209052

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Order

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

  • Frozen embryos. Delivered in 4 weeks (after paperwork in place). €1740*
  • Rederivation of mice from frozen stock, delivery time available upon request . €3880*

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
For this strain no provider MTA is needed. Distribution is based on the EMMA conditions only.

EMMA conditions
Legally binding conditions for the transfer

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