B6.129P2-Sp3tm1Sus/Cnrm

Status

Available to order

EMMA IDEM:02429
International strain nameB6.129P2-Sp3tm1Sus/Cnrm
Alternative nameSp3 knockout
Strain typeTargeted Mutant Strains : Knock-out
Allele/Transgene symbolSp3tm1Sus,
Gene/Transgene symbolSp3

Information from provider

ProviderSjaak Philipsen
Provider affiliationGenetics, Erasmus MC
Genetic informationThe targeting vector pBS-P-B-Delta-lacZ-neo-5'-Sp3 was designed to substitute exon 2 (amino acids 38-490) of the mouse Sp3 gene for IRES-LacZ-neo-polyA sequences. The lacZ-neo fusion gene is expressed under the control of the endogenous Sp3 promoter. The deleted sequences encode both glutamine-rich activation domains of Sp3. Thus, the disruption should result in a null allele.
Phenotypic informationSp3-deficient embryos are growth retarded and invariably die at birth of respiratory failure. Only minor morphological alterations were observed in the lung, and surfactant protein expression is indistinguishable from that of wild-type mice. Histological examinations of individual organs in Sp3-/- mice show a pronounced defect in late tooth formation. In Sp3 null mice, the dentin/enamel layer of the developing teeth is impaired due to the lack of ameloblast-specific gene products. Sp3 is also required for proper skeletal ossification. Both endochondral and intramembranous ossification are impaired in E18.5 Sp3-/- embryos. The absence of Sp3 also results in cell-autonomous hematopoietic defects, affecting in particular the erythroid and myeloid cell lineages. Finally, Sp3 is required for normal cardiac development; this is the likely cause of prenatal death of Sp3-/- embryos in the C57BL/6 background.
Breeding historyThe line was backcrossed to C57BL/6 for more than 20 generations.
References
  • Sp1/Sp3 compound heterozygous mice are not viable: impaired erythropoiesis and severe placental defects.;Krüger Imme, Vollmer Marion, Simmons David G, Elsässer Hans-Peter, Philipsen Sjaak, Suske Guntram, ;2007;Developmental dynamics : an official publication of the American Association of Anatomists;236;2235-44; 17584888
  • Transcription factor Sp3 knockout mice display serious cardiac malformations.;van Loo Pieter Fokko, Mahtab Edris A F, Wisse Lambertus J, Hou Jun, Grosveld Frank, Suske Guntram, Philipsen Sjaak, Gittenberger-de Groot Adriana C, ;2007;Molecular and cellular biology;27;8571-82; 17923686
  • Transcription factor Sp3 is essential for post-natal survival and late tooth development.;Bouwman P, Göllner H, Elsässer H P, Eckhoff G, Karis A, Grosveld F, Philipsen S, Suske G, ;2000;The EMBO journal;19;655-61; 10675334
  • Impaired ossification in mice lacking the transcription factor Sp3.;Göllner H, Dani C, Phillips B, Philipsen S, Suske G, ;2001;Mechanisms of development;106;77-83; 11472836
  • Impaired hematopoiesis in mice lacking the transcription factor Sp3.;Van Loo Pieter Fokko, Bouwman Peter, Ling Kam-Wing, Middendorp Sabine, Suske Guntram, Grosveld Frank, Dzierzak Elaine, Philipsen Sjaak, Hendriks Rudolf W, ;2003;Blood;102;858-66; 12676787

Information from EMMA

Archiving centreCNR, Consiglio Nazionale delle Ricerche, Monterotondo, Italy

Disease and phenotype information

MGI phenotypes (allele matching)
  • decreased body size / MGI
  • abnormal erythroid progenitor cell morphology / MGI
  • delayed bone ossification / MGI
  • abnormal interparietal bone morphology / MGI
  • abnormal supraoccipital bone morphology / MGI
  • abnormal nasal bone morphology / MGI
  • abnormal basisphenoid bone morphology / MGI
  • abnormal frontal bone morphology / MGI
  • abnormal parietal bone morphology / MGI
  • abnormal vertebral body morphology / MGI
  • abnormal xiphoid process morphology / MGI
  • abnormal erythropoiesis / MGI
  • abnormal cranium morphology / MGI
  • liver hypoplasia / MGI
  • small thymus / MGI
  • abnormal placenta labyrinth morphology / MGI
  • respiratory failure / MGI
  • abnormal tooth morphology / MGI
  • abnormal B cell differentiation / MGI
  • abnormal pulmonary alveolus morphology / MGI
  • abnormal ameloblast morphology / MGI
  • abnormal dentin morphology / MGI
  • abnormal bone mineralization / MGI
  • abnormal metatarsal bone morphology / MGI
  • abnormal metacarpal bone morphology / MGI
  • absent enamel / MGI
  • delayed endochondral bone ossification / MGI
  • delayed intramembranous bone ossification / MGI
  • abnormal sagittal suture morphology / MGI
  • abnormal presphenoid bone morphology / MGI
  • abnormal cervical atlas morphology / MGI
  • abnormal phalanx morphology / MGI
  • decreased CD4-positive, alpha beta T cell number / MGI
  • abnormal sternum ossification / MGI
  • abnormal osteoblast differentiation / MGI
  • abnormal trophoblast glycogen cell morphology / MGI
  • abnormal spongiotrophoblast cell morphology / MGI
  • decreased birth body size / MGI
  • abnormal basicranium morphology / MGI
  • neonatal lethality, complete penetrance / MGI
  • decreased spongiotrophoblast size / MGI

Literature references

  • Sp1/Sp3 compound heterozygous mice are not viable: impaired erythropoiesis and severe placental defects.;Krüger Imme, Vollmer Marion, Simmons David G, Elsässer Hans-Peter, Philipsen Sjaak, Suske Guntram, ;2007;Developmental dynamics : an official publication of the American Association of Anatomists;236;2235-44; 17584888
  • Transcription factor Sp3 knockout mice display serious cardiac malformations.;van Loo Pieter Fokko, Mahtab Edris A F, Wisse Lambertus J, Hou Jun, Grosveld Frank, Suske Guntram, Philipsen Sjaak, Gittenberger-de Groot Adriana C, ;2007;Molecular and cellular biology;27;8571-82; 17923686
  • Transcription factor Sp3 is essential for post-natal survival and late tooth development.;Bouwman P, Göllner H, Elsässer H P, Eckhoff G, Karis A, Grosveld F, Philipsen S, Suske G, ;2000;The EMBO journal;19;655-61; 10675334
  • Impaired ossification in mice lacking the transcription factor Sp3.;Göllner H, Dani C, Phillips B, Philipsen S, Suske G, ;2001;Mechanisms of development;106;77-83; 11472836
  • Impaired hematopoiesis in mice lacking the transcription factor Sp3.;Van Loo Pieter Fokko, Bouwman Peter, Ling Kam-Wing, Middendorp Sabine, Suske Guntram, Grosveld Frank, Dzierzak Elaine, Philipsen Sjaak, Hendriks Rudolf W, ;2003;Blood;102;858-66; 12676787

Information on how we integrate external resources can be found here

Order

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

  • Frozen embryos. Delivered in 4 weeks (after paperwork in place). €1740*
  • Frozen sperm. Delivered in 4 weeks (after paperwork in place). €1740*
  • Rederivation of mice from frozen stock, delivery time available upon request . €3880*

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
For this strain no provider MTA is needed. Distribution is based on the EMMA conditions only.

EMMA conditions
Legally binding conditions for the transfer

Other EMMA strains

Not found what you were looking for? Search here for other strains available from EMMA.


Search
INFRAFRONTIER® and European Mouse Mutant Archive - EMMA® are registered trademarks at the European Union Intellectual Property Office (EUIPO).