- spleen hypoplasia / MGI
- small testis / MGI
- postnatal growth retardation / MGI
- thymus hypoplasia / MGI
- reduced female fertility / MGI
- male infertility / MGI
- delayed vaginal opening / MGI
- small uterus / MGI
- homeostasis/metabolism phenotype / MGI
- hematopoietic system phenotype / MGI
- postnatal lethality, incomplete penetrance / MGI
B6.129P2-Sp4tm1Sus/Cnrm
Status | Available to order |
EMMA ID | EM:02431 |
International strain name | B6.129P2-Sp4tm1Sus/Cnrm |
Alternative name | Sp4 knockout |
Strain type | Targeted Mutant Strains : Knock-out |
Allele/Transgene symbol | Sp4tm1Sus, |
Gene/Transgene symbol | Sp4 |
Information from provider
Provider | Sjaak Philipsen |
Provider affiliation | Genetics, Erasmus MC |
Genetic information | A targeting vector was designed to replace sequences that encode the N-terminal activation domains of Sp4 protein (amino acids 4-557). This was obtained by replacing exons 2 and 3 of the mouse Sp4 gene by IRES-LacZ-polyA/PGK-neo sequences in the targeting vector. The lacZ gene is expressed under control of the endogenous Sp4 promoter. |
Phenotypic information | Increased mortality in the first weeks after birth, possibly due to cardiac arrest. Infertile; the animals are not interested in reproductive activities. Heterozygote Sp4 knockouts have no known phenotype. |
Breeding history | The line was backcrossed to C57BL/6 for more than 20 generations. |
References |
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Information from EMMA
Archiving centre | CNR, Consiglio Nazionale delle Ricerche, Monterotondo, Italy |
Disease and phenotype information
MGI phenotypes (allele matching)
Literature references
- Complex phenotype of mice homozygous for a null mutation in the Sp4 transcription factor gene.;Göllner H, Bouwman P, Mangold M, Karis A, Braun H, Rohner I, Del Rey A, Besedovsky H O, Meinhardt A, van den Broek M, Cutforth T, Grosveld F, Philipsen S, Suske G, ;2001;Genes to cells : devoted to molecular & cellular mechanisms;6;689-97; 11532028
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