B6.129P2-Sp4tm1Sus/Cnrm

Status

Available to order

EMMA IDEM:02431
International strain nameB6.129P2-Sp4tm1Sus/Cnrm
Alternative nameSp4 knockout
Strain typeTargeted Mutant Strains : Knock-out
Allele/Transgene symbolSp4tm1Sus,
Gene/Transgene symbolSp4

Information from provider

ProviderSjaak Philipsen
Provider affiliationGenetics, Erasmus MC
Genetic informationA targeting vector was designed to replace sequences that encode the N-terminal activation domains of Sp4 protein (amino acids 4-557). This was obtained by replacing exons 2 and 3 of the mouse Sp4 gene by IRES-LacZ-polyA/PGK-neo sequences in the targeting vector. The lacZ gene is expressed under control of the endogenous Sp4 promoter.
Phenotypic informationIncreased mortality in the first weeks after birth, possibly due to cardiac arrest. Infertile; the animals are not interested in reproductive activities. Heterozygote Sp4 knockouts have no known phenotype.
Breeding historyThe line was backcrossed to C57BL/6 for more than 20 generations.
References
  • Complex phenotype of mice homozygous for a null mutation in the Sp4 transcription factor gene.;Göllner H, Bouwman P, Mangold M, Karis A, Braun H, Rohner I, Del Rey A, Besedovsky H O, Meinhardt A, van den Broek M, Cutforth T, Grosveld F, Philipsen S, Suske G, ;2001;Genes to cells : devoted to molecular & cellular mechanisms;6;689-97; 11532028

Information from EMMA

Archiving centreCNR, Consiglio Nazionale delle Ricerche, Monterotondo, Italy

Disease and phenotype information

MGI phenotypes (allele matching)
  • spleen hypoplasia / MGI
  • small testis / MGI
  • postnatal growth retardation / MGI
  • thymus hypoplasia / MGI
  • reduced female fertility / MGI
  • male infertility / MGI
  • delayed vaginal opening / MGI
  • small uterus / MGI
  • homeostasis/metabolism phenotype / MGI
  • hematopoietic system phenotype / MGI
  • postnatal lethality, incomplete penetrance / MGI

Literature references

  • Complex phenotype of mice homozygous for a null mutation in the Sp4 transcription factor gene.;Göllner H, Bouwman P, Mangold M, Karis A, Braun H, Rohner I, Del Rey A, Besedovsky H O, Meinhardt A, van den Broek M, Cutforth T, Grosveld F, Philipsen S, Suske G, ;2001;Genes to cells : devoted to molecular & cellular mechanisms;6;689-97; 11532028

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Order

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

  • Frozen embryos. Delivered in 4 weeks (after paperwork in place). €1740*
  • Rederivation of mice from frozen stock, delivery time available upon request . €3880*

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
For this strain no provider MTA is needed. Distribution is based on the EMMA conditions only.

EMMA conditions
Legally binding conditions for the transfer

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