B6.129S6-Gt(ROSA)26Sortm1(Tva)Dsa/Cnrm
| Status | Available to order |
| EMMA ID | EM:02445 |
| International strain name | B6.129S6-Gt(ROSA)26Sortm1(Tva)Dsa/Cnrm |
| Alternative name | LSL-R26Tva-lacZ |
| Strain type | Targeted Mutant Strains : Knock-in |
| Allele/Transgene symbol | Gt(ROSA)26Sortm1(Tva)Dsa, |
| Gene/Transgene symbol | Gt(ROSA)26Sor |
Information from provider
| Provider | Dieter Saur |
| Provider affiliation | Technical University Munich |
| Genetic information | We generated a knock-in mouse line termed LSL-R26Tva-lacZ with concomitant conditional expression of the tumor virus A (TVA) receptor and nuclear lacZ, by targeting the Gt(ROSA)26Sor locus. A loxP flanked transcriptional stop cassette (LSL) was placed 5' of a TVA IRES LacZ-nls expression cassette. The LSL-TVA-IRES-lacZ cassette was inserted into the first intron of the Gt(ROSA)26Sor locus by homologous recombination in ES cells; cre-mediated recombination allows time- and site-specific expression of the TVA receptor for retroviral gene transfer using the RCAS system (replication-competent sarcoma-leukosis virus long terminal repeat with splice acceptor) and the lacZ reporter gene. To demonstrate the ability of this system for conditional somatic gene transfer in vivo, we directed TVA expression to the pancreas. Introduction of an RCASBP(A) vector (Bryan-RSV polymerase and subgroup A envelope viruses) carrying oncogenic KrasG12D induced focal ductal pancreatic lesions that recapitulate human pancreatic intraepithelial neoplasias, which progress to pancreatic ductal adenocarcinomas. |
| Phenotypic information | No defects. |
| Breeding history | 7 times backcrossed to C57BL/6 background. |
| References |
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Information from EMMA
| Archiving centre | CNR, Consiglio Nazionale delle Ricerche, Monterotondo, Italy |
| Animals used for archiving | homozygous C57BL/6, wild-type C57BL/6J |
| Stage of embryos | 2-cell |
Literature references
- An RCAS-TVA-based approach to designer mouse models.;Orsulic Sandra, ;2002;Mammalian genome : official journal of the International Mammalian Genome Society;13;543-7; 12420130
- A Cre-loxP-based mouse model for conditional somatic gene expression and knockdown in vivo by using avian retroviral vectors.;Seidler Barbara, Schmidt Annegret, Mayr Ulrich, Nakhai Hassan, Schmid Roland M, Schneider Günter, Saur Dieter, ;2008;Proceedings of the National Academy of Sciences of the United States of America;105;10137-42; 18621715