MGI phenotypes (allele matching)
B6.129P2-Gjd2tm4Kwi/Cnrm
| Status | Available to order |
| EMMA ID | EM:02510 |
| International strain name | B6.129P2-Gjd2tm4Kwi/Cnrm |
| Alternative name | Cx36flox(CFP) |
| Strain type | Targeted Mutant Strains : Conditional mutation |
| Allele/Transgene symbol | Gjd2tm4Kwi, |
| Gene/Transgene symbol | Gjd2 |
Information from provider
| Provider | Klaus WILLECKE |
| Provider affiliation | Molekulargenetik, Institut fuer Genetik, Universitaet Bonn |
| Genetic information | Instead of exon 1 and 2 of Cx36 (Gjd2) a loxP sites-flanked construct is inserted containing both exons and the intron of Cx36 and a frt sites-flanked neomycin selection cassette. After cre recombinase activity a cyan fluorescent protein reporter gene is expressed under the control of the endogenous Cx36 promoter. |
| Phenotypic information | No major defects were observed in the floxed state. After cre rembinase-mediated deletion homozygous Cx36 knock-out mice show visual transmission defects and reduced gamma oscillation. |
| Breeding history | After blastocyst injection the chimeras were bred with C57BL/6NCrl to obtain brown coat coloured offspring. This brown offspring was backcrossed to C57BL/6NCrl more than three times. |
| References |
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| Homozygous fertile | yes |
| Homozygous viable | yes |
| Homozygous matings required | no |
| Immunocompromised | no |
Information from EMMA
| Archiving centre | CNR, Consiglio Nazionale delle Ricerche, Monterotondo, Italy |
| Breeding at archiving centre | Backcrossed to C57BL/6J |
Disease and phenotype information
Literature references
- A new conditional mouse mutant reveals specific expression and functions of connexin36 in neurons and pancreatic beta-cells.;Wellershaus Kerstin, Degen Joachim, Deuchars Jim, Theis Martin, Charollais Anne, Caille Dorothée, Gauthier Benoit, Janssen-Bienhold Ulrike, Sonntag Stephan, Herrera Pedro, Meda Paolo, Willecke Klaus, ;2008;Experimental cell research;314;997-1012; 18258229
- Amacrine Cells Forming Gap Junctions With Intrinsically Photosensitive Retinal Ganglion Cells: ipRGC Types, Neuromodulator Contents, and Connexin Isoform.;Harrison Krystal R, Chervenak Andrew P, Resnick Sarah M, Reifler Aaron N, Wong Kwoon Y, ;2021;Investigative ophthalmology & visual science;62;10; 33410914