MGI phenotypes (allele matching)
B6.129S7-Aox4tm1Enga/Cnrm
| Status | Available to order |
| EMMA ID | EM:02590 |
| International strain name | B6.129S7-Aox4tm1Enga/Cnrm |
| Alternative name | C57BL/6-AOH2_KO |
| Strain type | Targeted Mutant Strains : Knock-out |
| Allele/Transgene symbol | Aox4tm1Enga, |
| Gene/Transgene symbol | Aox4 |
Information from provider
| Provider | Enrico Garattini |
| Provider affiliation | Biochimica e farmacologia molecolare, Istituto di Ricerche Farmacologiche "Mario Negri" |
| Genetic information | Targeting construct is derived from 129/Sv DNA. Exon 22 and surrounding intron sequences were replaced by the neomycin resistance gene. |
| Phenotypic information | Knock-out animals are viable and fertile, although they have an absolute deficit of retinaldehyde oxidase activity in the Harderian gland, decreased retinoic acid levels, and down-regulation of retinoid-dependent genes. Moreover they show thickening of the epidermis in basal conditions and after UV light exposure. |
| Breeding history | The knock-out line was obtained upon ES cell injection in B6D2F1 blastocyst and successively backcrossed to C57BL/6N for 9 generations. Heterozygous animals were maintained on C57BL/6N background in SPF facility. |
| References |
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| Homozygous fertile | yes |
| Homozygous viable | yes |
| Homozygous matings required | no |
| Immunocompromised | no |
Information from EMMA
| Archiving centre | CNR, Consiglio Nazionale delle Ricerche, Monterotondo, Italy |
| Breeding at archiving centre | Backcrossed to C57BL/6J |
Disease and phenotype information
Literature references
- Role of the molybdoflavoenzyme aldehyde oxidase homolog 2 in the biosynthesis of retinoic acid: generation and characterization of a knockout mouse.;Terao Mineko, Kurosaki Mami, Barzago Maria Monica, Fratelli Maddalena, Bagnati Renzo, Bastone Antonio, Giudice Chiara, Scanziani Eugenio, Mancuso Alessandra, Tiveron Cecilia, Garattini Enrico, ;2009;Molecular and cellular biology;29;357-77; 18981221