B6.129P2-Gjc1tm1Kwi/Cnrm
| Status | Available to order |
| EMMA ID | EM:00331 |
| International strain name | B6.129P2-Gjc1tm1Kwi/Cnrm |
| Alternative name | Cx45 KO (LacZ) |
| Strain type | Targeted Mutant Strains : Knock-out |
| Allele/Transgene symbol | Gjc1tm1Kwi, |
| Gene/Transgene symbol | Gjc1 |
Information from provider
| Provider | Klaus WILLECKE |
| Provider affiliation | Molekulargenetik, Institut fuer Genetik, Universitaet Bonn |
| Genetic information | Exon 3, including the whole open reading frame of Cx45 (Gjc1), is homologously replaced by the lacZ open reading frame. The selection marker gene (neo) is still in the genome. |
| Phenotypic information | Embryonic lethal at 10.5 dpc, caused by endothelium malformation. |
| Breeding history | After blastocyst injection the chimeras were bred with C57BL/6NCrl to obtain brown coat coloured offspring. This brown offspring was backcrossed to C57BL/6NCrl more than three times. |
| References |
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Information from EMMA
| Archiving centre | CNR, Consiglio Nazionale delle Ricerche, Monterotondo, Italy |
| Breeding at archiving centre | Backcrossed to C57BL/6J |
Literature references
- Defective vascular development in connexin 45-deficient mice.;Krüger O, Plum A, Kim J S, Winterhager E, Maxeiner S, Hallas G, Kirchhoff S, Traub O, Lamers W H, Willecke K, ;2000;Development (Cambridge, England);127;4179-93; 10976050