B6.Cg-Tg(tetO-MYC,-OVAL)#Gtgm/Ieg
| Status | Available to order |
| EMMA ID | EM:04319 |
| Citation information | RRID:IMSR_EM:04319 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information. |
| International strain name | B6.Cg-Tg(tetO-MYC,-OVAL)#Gtgm/Ieg |
| Alternative name | c-myc/OVA |
| Strain type | Transgenic Strains |
| Allele/Transgene symbol | Tg(tetO-MYC,-OVAL)#Gtgm |
| Gene/Transgene symbol | Tg(tetO-MYC,-OVAL)#Gtgm |
Information from provider
| Provider | Ines Gütgemann |
| Provider affiliation | Pathology, University of Bonn |
| Genetic information | C-myc/ovalbumin (OVA) transgenic mice were generated using a bidirectional cytomegalovirus tetracycline-controlled transactivator containing plasmid, pBI-4.11. On one side, the human c-myc complementary DNA, exons 2 and 3, was introduced using NotI and SalI and OVA encoding complementary DNA on the other side using MluI and NheI restriction sites. C-myc/OVA transgenic mice were generated by nuclear microinjection of linearized plasmid DNA into the male pronucleus of fertilized mouse oocytes derived from C57BL/6J x (C57BL/6J x DBA/2)F1 mice or C57BL/6J mice by standard techniques. C-myc/OVA mice were back-crossed to the C57BL/6J background for at least twelve generations. |
| Phenotypic information | If crossed to LAPtTA (liver-enriched activator protein tetracycline-controlled transactivator) transgenic mice, double transgenic mice develop multifocal hepatocellular carcinomas in a time dependent and tetracycline dependent manner. Cytosolic ovalbumin expression is found within tumor cells upon transgene induction. |
| Breeding history | FELASA conditions. More than 10 generations backcrossed to C57BL/6J. |
| References |
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| Homozygous fertile | not known |
| Homozygous viable | no |
| Homozygous matings required | no |
| Immunocompromised | no |
Information from EMMA
| Archiving centre | Helmholtz Zentrum Muenchen - German Research Center for Environmental Health (GmbH), Oberschleißheim, Germany |
| Animals used for archiving | heterozygous C57BL/6J males, wild-type C57BL/6J females |
| Stage of embryos | 2-cell |
Literature references
- Autochthonous liver tumors induce systemic T cell tolerance associated with T cell receptor down-modulation.;Ney Jasmin T, Schmidt Thomas, Kurts Christian, Zhou Qi, Eckert Dawid, Felsher Dean W, Schorle Hubert, Knolle Percy, Tüting Thomas, Barchet Winfried, Büttner Reinhard, Limmer Andreas, Gütgemann Ines, ;2009;Hepatology (Baltimore, Md.);49;471-81; 19105207