FVB-Tg(tetO-Chrnb2*V287L)H3Gica/Cnrm
| Status | Available to order |
| EMMA ID | EM:04321 |
| Citation information | RRID:IMSR_EM:04321 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information. |
| International strain name | FVB-Tg(tetO-Chrnb2*V287L)H3Gica/Cnrm |
| Alternative name | Tg(TRE-Chrnb2VL)/H3 |
| Strain type | Transgenic Strains |
| Allele/Transgene symbol | Tg(tetO-Chrnb2*V287L)H3Gica |
| Gene/Transgene symbol | Tg(tetO-Chrnb2*V287L)H3Gica |
Information from provider
| Provider | Giorgio Casari |
| Provider affiliation | San Raffaele University and San Raffaele Scientific Institute |
| Genetic information | The transgenic line expresses the ADNFLE mutation V287L of the Chrnb2 gene (cholinergic receptor, nicotinic, beta polypeptide 2, neuronal) under the Tet-Off promoter, when crossed with tetracycline-controlled transactivator protein (tTA)-expressing strain. The H3 lines carries 4 copies of the transgene. |
| Phenotypic information | Mice expressing ADNFLE mutation display epileptic phenotype with interictal spikes and spontaneous seizure associated to delayed desensitization of the nicotinic receptor. H3 line carries 4 copies of the transgene and expresses mutant beta2 protein at high levels. This overexpression does not influence the total number of beta2 containing receptors, but determines a more severe epileptic phenotype, with frequent spontaneous seizures, probably due to the higher probability of incorporation of mutant subunits into functional receptors, which are pentamers of alpha and beta subunits. |
| Breeding history | H3 strain is FVB inbred. Four copies of the transgene are tandemly inserted in a single locus. Transgenic mice are maintained by crossing with wild-type FVB/N inbred mice. Progeny inherits transgene with the expected Mendelian 0.5 ratio. Hemizygous transgenic mice are viable and fertile. |
| References |
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| Homozygous fertile | not known |
| Homozygous viable | not known |
| Homozygous matings required | no |
| Immunocompromised | no |
Information from EMMA
| Archiving centre | CNR, Consiglio Nazionale delle Ricerche, Monterotondo, Italy |
| Animals used for archiving | heterozygous FVB/N males, wild-type FVB/N females |
| Stage of embryos | 2-cell |
Literature references
- Expression of mutant beta2 nicotinic receptors during development is crucial for epileptogenesis.;Manfredi Irene, Zani Alessia D, Rampoldi Luca, Pegorini Simona, Bernascone Ilenia, Moretti Milena, Gotti Cecilia, Croci Laura, Consalez G Giacomo, Ferini-Strambi Luigi, Sala Mariaelvina, Pattini Linda, Casari Giorgio, ;2009;Human molecular genetics;18;1075-88; 19153075