B6;SJL-Tg(Tek-cre)1Ywa/Cnbc

Status	Available to order
EMMA ID	EM:04325
International strain name	B6;SJL-Tg(Tek-cre)1Ywa/Cnbc
Alternative name	Tie 2 Cre
Strain type	Transgenic Strains
Allele/Transgene symbol	Tg(Tek-cre)1Ywa,
Gene/Transgene symbol	Tg(Tek-cre)1Ywa

Information from provider

Provider	Masashi Yanagisawa
Provider affiliation	Psychiatry, University of Texas Southwestern Medical Center
Additional owner	Strain provided by University of Texas Southwestern Medical Center, Dallas TX, U.S.A.
Genetic information	In Tie2Cre mice, Cre recombinase expression is tightly regulated under the control of endothelial specific Tie2 promoter and enhancer. The specific expression of Cre recombinase in endothelial cells is verified by crossing Tie2Cre mice with loxP-based reporter mice.
Phenotypic information	In Tie2Cre mice, Cre recombinase expression is tightly regulated under the control of endothelial specific Tie2 promoter and enhancer. The specific expression of Cre recombinase in endothelial cells is verified by crossing Tie2Cre mice with loxP-based reporter mice.
Breeding history	Backcrossed to C57BL/6 an unknown number of times.
References	Tie2-Cre transgenic mice: a new model for endothelial cell-lineage analysis in vivo.;Kisanuki Y Y, Hammer R E, Miyazaki J, Williams S C, Richardson J A, Yanagisawa M, ;2001;Developmental biology;230;230-42; 11161575 Uniform vascular-endothelial-cell-specific gene expression in both embryonic and adult transgenic mice.;Schlaeger T M, Bartunkova S, Lawitts J A, Teichmann G, Risau W, Deutsch U, Sato T N, ;1997;Proceedings of the National Academy of Sciences of the United States of America;94;3058-63; 9096345
Homozygous fertile	yes
Homozygous viable	yes
Homozygous matings required	no
Immunocompromised	no

Information from EMMA

Archiving centre	CNB-CSIC, Centro Nacional de Biotecnologia, Madrid, Spain
Animals used for archiving	heterozygous C57BL/6J, wild-type C57BL/6J
Stage of embryos	2-cell

Literature references

Tie2-Cre transgenic mice: a new model for endothelial cell-lineage analysis in vivo.;Kisanuki Y Y, Hammer R E, Miyazaki J, Williams S C, Richardson J A, Yanagisawa M, ;2001;Developmental biology;230;230-42; 11161575
Uniform vascular-endothelial-cell-specific gene expression in both embryonic and adult transgenic mice.;Schlaeger T M, Bartunkova S, Lawitts J A, Teichmann G, Risau W, Deutsch U, Sato T N, ;1997;Proceedings of the National Academy of Sciences of the United States of America;94;3058-63; 9096345

Order

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

Frozen embryos. Delivered in 4 weeks (after paperwork in place). €1740^*
Frozen sperm. Delivered in 4 weeks (after paperwork in place). €1740^*
Rederivation of mice from frozen stock, delivery time available upon request . €3880^*

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

^* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Genotyping protocol

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
For this strain no provider MTA is needed. Distribution is based on the EMMA conditions only.

EMMA conditions
Legally binding conditions for the transfer

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