B6;SJL-Tg(Mt1-SERPINE1)1Upj/Cnrm
| Status | Available to order |
| EMMA ID | EM:04468 |
| International strain name | B6;SJL-Tg(Mt1-SERPINE1)1Upj/Cnrm |
| Alternative name | PAI-1 |
| Strain type | Transgenic Strains |
| Allele/Transgene symbol | Tg(Mt1-SERPINE1)1Upj, |
| Gene/Transgene symbol | Tg(Mt1-SERPINE1)1Upj |
Information from provider
| Provider | Andreina Poggi |
| Provider affiliation | Consorzio Mario Negri Sud |
| Additional owner | Dr. Laurence A. Erickson and Dr. Keith R. Marotti, Pfizer (former Upjohn), Kalamazoo, MI, U.S.A. |
| Genetic information | The DNA construct used in the production of SERPINE1 (PAI-1) transgenic mice contained the metallothionein I promoter, human endothelial cell SERPINE1 complementary DNA, and the bovine growth hormone polyadenylation sequence. |
| Phenotypic information | Mice putatively transgenic for human SERPINE1 (PAI-1) showed a single, early indication of disturbance of the haemostatic system. A distinct area of subcutaneous haemorrhage was apparent at tail's tip of mice at 3 days after birth, followed by necrosis and swollen hind feet at about 12 days, as a result of venous occlusion and oedema. At about 2 weeks of age, the necrotic portion of the tail completely sloughed off, whereas the swollen hind limbs appeared normal. |
| Breeding history | SERPINE1 (PAI-1) transgenic mice originally obtained from Dr. L.A. Erickson, The Upjohn Company, Kalamazoo, Michigan USA, were backcrossed for 4 generations to C57BL/6 mice; mice were subsequently intercrossed (brother x sister) for more than 100 generations. |
| References |
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| Homozygous fertile | yes |
| Homozygous viable | yes |
| Homozygous matings required | no |
| Immunocompromised | not known |
Information from EMMA
| Archiving centre | CNR, Consiglio Nazionale delle Ricerche, Monterotondo, Italy |
Literature references
- Development of venous occlusions in mice transgenic for the plasminogen activator inhibitor-1 gene.;Erickson L A, Fici G J, Lund J E, Boyle T P, Polites H G, Marotti K R, ;1990;Nature;346;74-6; 2366866
- Immunohistochemical localization of fibrinolytic proteins in tissues from human PAI gene transgenic mice;Salvatore L, Iacoviello L, Bellelli E, Poggi A, Donati MB;1995;Thrombosis and Haemostasis (Abstracts);73;1005;
- A simple and reproducible model of arterial injury in normal and PAI-1 transgenic mice;Di Sciullo A, Mironov A, Nasuti M, Di Nardo P, Poggi A;1995;Thrombosis and Haemostasis (Abstracts);73;1333;