B6Dnk;B6Brd;B6N-Tyr^c-Brd Phf20^{tm1a(EUCOMM)Wtsi}/WtsiCnbc

Status	Available to order
EMMA ID	EM:04476
Citation information	RRID:IMSR_EM:04476 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information.
International strain name	B6Dnk;B6Brd;B6N-Tyr^c-Brd Phf20^{tm1a(EUCOMM)Wtsi}/WtsiCnbc
Alternative name	EPD0027_2_G07
Strain type	Targeted Mutant Strains
Allele/Transgene symbol	Phf20^{tm1a(EUCOMM)Wtsi}
Gene/Transgene symbol	Phf20
Disclaimer	Please note that for EUCOMM and KOMP-CSD mice supplied to the scientific community by INFRAFRONTIER/EMMA: We can not guarantee a null mutation for Knock-out first alleles (tm1a alleles, see http://www.mousephenotype.org/about-ikmc/targeting-strategies) as the critical exon has not been deleted. That the structure of the targeted mutation in the ES cells obtained from EUCOMM/KOMP to generate EUCOMM/KOMP mice is not verified by INFRAFRONTIER/EMMA. It is recommended that the recipient confirms the mutation structure. No check for determining the copy number of the targeting construct in ES cells obtained from EUCOMM/KOMP is done by INFRAFRONTIER/EMMA. The level of quality control before mice are released is to confirm the individual mouse genotype by short range PCR.

Information from provider

Provider	Wellcome Trust Sanger Institute
Provider affiliation	Wellcome Trust Sanger Institute
Genetic information	This mouse line originates from EUCOMM ES clone EPD0027_2_G07. For further details on the construction of this clone see the page at the IMPC portal.
Phenotypic information	Potential phenotyping data in the IMPC portal
References	None available

Information from EMMA

Archiving centre	CNB-CSIC, Centro Nacional de Biotecnologia, Madrid, Spain

Disease and phenotype information

IMPC phenotypes (allele matching)

preweaning lethality, incomplete penetrance / IMPC
developmental and structural abnormality / IMPC

IMPC phenotypes (gene matching)

preweaning lethality, incomplete penetrance / IMPC
developmental and structural abnormality / IMPC

MGI phenotypes (gene matching)

delayed bone ossification / MGI
fragile skeleton / MGI
decreased bone marrow cell number / MGI
small spleen / MGI
small thymus / MGI
decreased thymocyte number / MGI
decreased body weight / MGI
decreased body size / MGI
thymus hypoplasia / MGI
abnormal T cell differentiation / MGI
abnormal hematopoietic system morphology/development / MGI
decreased fetal size / MGI
decreased lumbar vertebrae number / MGI
increased double-negative T cell number / MGI
decreased double-positive T cell number / MGI
intermingled spleen red and white pulp / MGI
decreased total body fat amount / MGI
neonatal lethality, incomplete penetrance / MGI

Information on how we integrate external resources can be found here

Order

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

Frozen embryos. Delivered in 4 weeks (after paperwork in place). €1740^*
Rederivation of mice from frozen stock, delivery time available upon request . €3880^*
LacZ tagged null allele (tm1b allele), service fee and delivery time available upon request
Conditional ready allele (tm1c allele), service fee and delivery time available upon request
Reporterless null allele (tm1d allele), service fee and delivery time available upon request

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

^* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Genotyping protocol

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
Distribution of this strain is subject to a provider MTA. Both signing of the MTA and submission of the online EMMA Mutant Request Form are required before material can be shipped.

EMMA conditions
Legally binding conditions for the transfer

Other EMMA strains

Not found what you were looking for? Search here for other strains available from EMMA.

INFRAFRONTIER^® and European Mouse Mutant Archive - EMMA^® are registered trademarks at the European Union Intellectual Property Office (EUIPO).

B6Dnk;B6Brd;B6N-Tyrc-Brd Phf20tm1a(EUCOMM)Wtsi/WtsiCnbc