FVB(129S7)-Tg(Prnp/PRNP*AHQ)EF50Pcg/H

Status	Available to order
EMMA ID	EM:04554
Citation information	RRID:IMSR_EM:04554 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information.
International strain name	FVB(129S7)-Tg(Prnp/PRNP*AHQ)EF50Pcg/H
Alternative name	Tg(OvPrP)EF50
Strain type	Transgenic Strains
Allele/Transgene symbol	Tg(Prnp/PRNP*AHQ)EF50Pcg
Gene/Transgene symbol	Tg(Prnp/PRNP*AHQ)EF50Pcg

Information from provider

Provider	Peter Griffiths
Provider affiliation	Department of Pathology and Host Susceptibility, Animal Health and Veterinary Laboratories Agency (AHVLA)
Genetic information	A ~12 kb transgene, comprised of chimaeric DNA sequence encoding a sheep PrP (AHQ allele) coding region and mouse PrP gene promoter and 3' UTR, was used to microinject pronuclear stage FVB/N fertilised embryos.
Phenotypic information	Overexpression of transgenic ovine PrPC in brain and other tissues of this mouse line in order to confer increased susceptibility to TSE diseases compared to wild-type mice.
Breeding history	Inbred to 7 generations. Brother and sister matings.
References	Overexpression of chimaeric murine/ovine PrP (A136H154Q171) in transgenic mice facilitates transmission and differentiation of ruminant prions.;Griffiths Peter C, Plater Jane M, Chave Alun, Jayasena Dhanushka, Tout Anna C, Rice Paul B, Vickery Christopher M, Spiropoulos John, Stack Michael J, Windl Otto, ;2013;The Journal of general virology;94;2577-2586; 23761404
Homozygous fertile	yes
Homozygous viable	yes
Homozygous matings required	yes
Immunocompromised	not known

Information from EMMA

Archiving centre	Mary Lyon Centre at MRC Harwell, Oxford, United Kingdom
Animals used for archiving	homozygous males, homozygous females
Breeding at archiving centre	None

Literature references

Overexpression of chimaeric murine/ovine PrP (A136H154Q171) in transgenic mice facilitates transmission and differentiation of ruminant prions.;Griffiths Peter C, Plater Jane M, Chave Alun, Jayasena Dhanushka, Tout Anna C, Rice Paul B, Vickery Christopher M, Spiropoulos John, Stack Michael J, Windl Otto, ;2013;The Journal of general virology;94;2577-2586; 23761404

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Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

Frozen embryos. Delivered in 4 weeks (after paperwork in place). €1740^*
Rederivation of mice from frozen stock, delivery time available upon request . €3880^*
Tissue - Types of tissue, service fee and delivery time available upon request

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

^* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

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Practical information

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