C57BL/6J-Col8a2Aca23/Ieg

Status

Available to order

EMMA IDEM:04573
Citation informationRRID:IMSR_EM:04573 

Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information.

 
International strain nameC57BL/6J-Col8a2Aca23/Ieg
Alternative nameAca23
Strain typeInduced Mutant Strains : Chemically-induced
Allele/Transgene symbolCol8a2Aca23
Gene/Transgene symbolCol8a2

Information from provider

ProviderOliver Puk
Provider affiliationInstitut fuer Entwicklungsgenetik, AG Graw, Helmholtz Zentrum Muenchen
Genetic informationENU induced Col8a2 G257D missense mutation.

Disclaimer - Special restrictions:

1) Mutant development was phenotype driven. Although for some mutant lines, linkage data and the molecular characterization of the causative mutation has been published, EMMA has not genotyped the strains and can not provide genotyping protocols.
2) Some lines showed low penetrance of the phenotype.
3) Only sperm available. Rederivation service can not be offered.
4) EMMA quality control standards may not apply for these strains.
5) EMMA has not verified the breeding performance and the genetic background of the strains.

The genotype/phenotype of the mice is not confirmed by INFRAFRONTIER/EMMA. We strongly recommend that the recipient confirms genotype/phenotype upon receipt.
Phenotypic informationAca23 is characterized by posterior corneal dystrophies and keratoglobus.
Breeding historyIncross, 10 generations
ReferencesNone available
Homozygous fertileyes
Homozygous viableyes
Homozygous matings requiredno
Immunocompromisedno

Information from EMMA

Archiving centreHelmholtz Zentrum Muenchen - German Research Center for Environmental Health (GmbH), Oberschleißheim, Germany
Animals used for archivinghomozygous C57BL/6J males

Disease and phenotype information

Orphanet associated rare diseases, based on orthologous gene matching

MGI phenotypes (allele matching)
  • abnormal eye morphology / MGI
  • decreased corneal stroma thickness / MGI
  • abnormal Descemet membrane / MGI
  • abnormal eye anterior chamber morphology / MGI
  • vision/eye phenotype / MGI
  • decreased cornea thickness / MGI
  • abnormal corneal epithelium morphology / MGI
  • enlarged eye anterior chamber / MGI
MGI phenotypes (gene matching)
  • abnormal eye morphology / MGI
  • decreased corneal stroma thickness / MGI
  • abnormal Descemet membrane / MGI
  • abnormal eye anterior chamber morphology / MGI
  • abnormal corneal endothelium morphology / MGI
  • vision/eye phenotype / MGI
  • decreased cornea thickness / MGI
  • abnormal corneal epithelium morphology / MGI
  • abnormal mitochondrion morphology / MGI
  • increased apoptosis / MGI
  • abnormal autophagy / MGI
  • abnormal endoplasmic reticulum morphology / MGI
  • enlarged eye anterior chamber / MGI

Information on how we integrate external resources can be found here

Order

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

  • Frozen sperm. Delivered in 4 weeks (after paperwork in place). €1740*
  • Rederivation of mice from frozen stock, delivery time available upon request . €3880*

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Genotyping protocol

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
Distribution of this strain is subject to a provider MTA. Both signing of the MTA and submission of the online EMMA Mutant Request Form are required before material can be shipped.

EMMA conditions
Legally binding conditions for the transfer

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