C57BL/6NTac-Col4a5tm1a(EUCOMM)Wtsi/Cnrm

Status

Available to order

EMMA IDEM:04732
Citation informationRRID:IMSR_EM:04732 

Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information.

 
International strain nameC57BL/6NTac-Col4a5tm1a(EUCOMM)Wtsi/Cnrm
Alternative nameEPD0233_5_G04
Strain typeTargeted Mutant Strains
Allele/Transgene symbolCol4a5tm1a(EUCOMM)Wtsi
Gene/Transgene symbolCol4a5
DisclaimerPlease note that for EUCOMM and KOMP-CSD mice supplied to the scientific community by INFRAFRONTIER/EMMA:
  1. We can not guarantee a null mutation for Knock-out first alleles (tm1a alleles, see http://www.mousephenotype.org/about-ikmc/targeting-strategies) as the critical exon has not been deleted.
  2. That the structure of the targeted mutation in the ES cells obtained from EUCOMM/KOMP to generate EUCOMM/KOMP mice is not verified by INFRAFRONTIER/EMMA. It is recommended that the recipient confirms the mutation structure.
  3. No check for determining the copy number of the targeting construct in ES cells obtained from EUCOMM/KOMP is done by INFRAFRONTIER/EMMA.
  4. The level of quality control before mice are released is to confirm the individual mouse genotype by short range PCR.

Information from provider

Provider CNR, Consiglio Nazionale delle Ricerche
Provider affiliationEMMA-Monterotondo Campus International Development, CNR, Consiglio Nazionale delle Ricerche
Genetic informationThis mouse line originates from EUCOMM ES clone EPD0233_5_G04. For further details on the construction of this clone see the page at the IMPC portal.
Phenotypic informationPotential phenotyping data in the IMPC portal
ReferencesNone available

Information from EMMA

Archiving centreCNR, Consiglio Nazionale delle Ricerche, Monterotondo, Italy
Animals used for archivingheterozygous C57BL/6NTac males, wild-type C57BL/6NTac females
Stage of embryos2-cell

Disease and phenotype information

Orphanet associated rare diseases, based on orthologous gene matching

IMPC phenotypes (gene matching)
  • increased spleen weight / IMPC
  • increased circulating calcium level / IMPC
  • decreased mean corpuscular hemoglobin / IMPC
  • increased circulating amylase level / IMPC
  • impaired glucose tolerance / IMPC
  • increased circulating creatinine level / IMPC
  • abnormal lens morphology / IMPC
  • decreased hemoglobin content / IMPC
  • increased blood urea nitrogen level / IMPC
  • decreased total body fat amount / IMPC
  • decreased circulating serum albumin level / IMPC
  • increased circulating cholesterol level / IMPC
  • increased leukocyte cell number / IMPC
  • increased circulating triglyceride level / IMPC
  • decreased circulating fructosamine level / IMPC
  • increased large unstained cell number / IMPC
  • increased neutrophil cell number / IMPC
  • decreased mean corpuscular volume / IMPC
  • thrombocytosis / IMPC
  • decreased hematocrit / IMPC
  • decreased circulating total protein level / IMPC
  • abnormal kidney morphology / IMPC
  • decreased erythrocyte cell number / IMPC
  • increased red blood cell distribution width / IMPC
  • persistence of hyaloid vascular system / IMPC
  • increased circulating phosphate level / IMPC
  • increased circulating HDL cholesterol level / IMPC
MGI phenotypes (gene matching)
  • premature death / MGI
  • abnormal kidney morphology / MGI
  • abnormal renal tubule morphology / MGI
  • increased urine protein level / MGI
  • abnormal lens capsule morphology / MGI
  • pale kidney / MGI
  • abnormal eye physiology / MGI
  • abnormal renal glomerulus morphology / MGI
  • increased blood urea nitrogen level / MGI

Information on how we integrate external resources can be found here

Order

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

  • Frozen embryos. Delivered in 4 weeks (after paperwork in place). €1740*
  • Frozen sperm. Delivered in 4 weeks (after paperwork in place). €1740*
  • Rederivation of mice from frozen stock, delivery time available upon request . €3880*

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Genotyping protocol

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
Distribution of this strain is subject to a provider MTA. Both signing of the MTA and submission of the online EMMA Mutant Request Form are required before material can be shipped.

EMMA conditions
Legally binding conditions for the transfer

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