B6JOlaHsd.Cg-Cdkn2atm1Rdp/Cnbc

Status

Available to order

EMMA IDEM:04766
International strain nameB6JOlaHsd.Cg-Cdkn2atm1Rdp/Cnbc
Alternative nameInk4a-Arf (-/-) null KO mice (P16 KO), Tyrp1<+>
Strain typeTargeted Mutant Strains : Knock-out
Allele/Transgene symbolCdkn2atm1Rdp,
Gene/Transgene symbolCdkn2a

Information from provider

ProviderManuel Serrano
Provider affiliationMolecular Oncology, CNIO (Spanish National Cancer Research Centre)
Additional ownerLluis Montoliu, CNB-CSIC, Madrid, Spain
Genetic informationKnock-out of cyclin-dependent kinase inhibitor 2A (Cdkn2a or Ink4a-Arf) locus. Insertion, intragenic deletion: exons E2 and E3 were deleted and replaced by a neomycin cassette. Note that both the p16Ink4a and p19ARF alternative splice transcripts are mutated in this Knock-out allele. Initially associated with the Tyrp1b/Tyrp1b "brown" mutant allele, on the same chromosome, but eventually segregated to Tyrp1+ allele, through spontaneous recombination, and backcrossed to C57BL/6JOlaHsd, where it has been maintained since, at least, 2002.
Phenotypic informationThe mice are phenotypically black coat coloured (nonagouti, a/a) and viable, but develop spontaneous tumors at an early age (3 months for homozygous mutants, 6 months for heterozygous mutants) and are highly sensitive to carcinogenic treatments.
Breeding historyAfter recombining out the Tyrp1b mutant allele, backcrossed again to C57BL/6JOlaHsd and maintained in this inbred strain since, at least, 2002.
References
  • A strategy to study tyrosinase transgenes in mouse melanocytes.;Lavado Alfonso, Matheu Ander, Serrano Manuel, Montoliu Lluís, ;2005;BMC cell biology;6;18; 15826307
  • Role of the INK4a locus in tumor suppression and cell mortality.;Serrano M, Lee H, Chin L, Cordon-Cardo C, Beach D, DePinho R A, ;1996;Cell;85;27-37; 8620534
Homozygous fertileyes
Homozygous viableyes
Homozygous matings requiredno
Immunocompromisedno

Information from EMMA

Archiving centreCNB-CSIC, Centro Nacional de Biotecnologia, Madrid, Spain
Animals used for archivingheterozygous C57BL/6JOlaHsd, wild-type C57BL/6JOlaHsd
Stage of embryos8-cell

Disease and phenotype information

MGI phenotypes (allele matching)
  • abnormal retinal photoreceptor morphology / MGI
  • microphthalmia / MGI
  • cataract / MGI
  • abnormal posterior eye segment morphology / MGI
  • abnormal lens development / MGI
  • abnormal retinal neuronal layer morphology / MGI
  • persistent hyperplastic primary vitreous / MGI
  • increased brain tumor incidence / MGI
  • extramedullary hematopoiesis / MGI
  • abnormal spleen morphology / MGI
  • increased tumor incidence / MGI
  • increased B cell derived lymphoma incidence / MGI
  • increased sarcoma incidence / MGI
  • abnormal spleen red pulp morphology / MGI
  • abnormal cell cycle / MGI
  • increased hemangiosarcoma incidence / MGI
  • increased spleen white pulp amount / MGI
  • increased cutaneous melanoma incidence / MGI
  • increased fibrosarcoma incidence / MGI
  • increased fibroblast proliferation / MGI
  • abnormal keratinocyte physiology / MGI
  • increased squamous cell carcinoma incidence / MGI
  • abnormal skin physiology / MGI
  • abnormal cell physiology / MGI
  • delayed cellular replicative senescence / MGI
  • premature death / MGI
  • increased lymphoma incidence / MGI

Literature references

  • A strategy to study tyrosinase transgenes in mouse melanocytes.;Lavado Alfonso, Matheu Ander, Serrano Manuel, Montoliu Lluís, ;2005;BMC cell biology;6;18; 15826307
  • Role of the INK4a locus in tumor suppression and cell mortality.;Serrano M, Lee H, Chin L, Cordon-Cardo C, Beach D, DePinho R A, ;1996;Cell;85;27-37; 8620534

Information on how we integrate external resources can be found here

Order

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

  • Frozen embryos. Delivered in 4 weeks (after paperwork in place). €1740*
  • Rederivation of mice from frozen stock, delivery time available upon request . €3880*

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Genotyping protocol

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
For this strain no provider MTA is needed. Distribution is based on the EMMA conditions only.

EMMA conditions
Legally binding conditions for the transfer

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