B6;129S4-Cers2Gt(S16-4B1)Sor/Cnrm

Status

Available to order

EMMA IDEM:04933
International strain nameB6;129S4-Cers2Gt(S16-4B1)Sor/Cnrm
Alternative nameCerS2 gene trap
Strain typeGene-trap
Allele/Transgene symbolCers2Gt(S16-4B1)Sor,
Gene/Transgene symbolCers2

Information from provider

ProviderKlaus WILLECKE
Provider affiliationMolekulargenetik, Institut fuer Genetik, Universitaet Bonn
Genetic informationA gene-trap (Rosafary) is inserted into intron 1 of Cers2 (Lass2), leading to the disruption of Cers2 expression. Instead, a lacZ reporter gene is expressed under control of the Cers2 promoter. The hygromycin resistance cassette was removed via flp recombinase-mediated recombination.
Phenotypic informationAdult homozygous Cers2 gene-trap mice are smaller than their wild-type littermates. They develop liver carcinomas and show myelin degeneration.
Breeding historyThe first heterozygous offspring of chimeras were backcrossed three times with C57BL/6. Cers2 gene-trap mice are currently mated to C57BL/6 or inbred.
References
  • Adult ceramide synthase 2 (CERS2)-deficient mice exhibit myelin sheath defects, cerebellar degeneration, and hepatocarcinomas.;Imgrund Silke, Hartmann Dieter, Farwanah Hany, Eckhardt Matthias, Sandhoff Roger, Degen Joachim, Gieselmann Volkmar, Sandhoff Konrad, Willecke Klaus, ;2009;The Journal of biological chemistry;284;33549-60; 19801672
Homozygous fertilenot known
Homozygous viableyes
Homozygous matings requiredno
Immunocompromisedno

Information from EMMA

Archiving centreCNR, Consiglio Nazionale delle Ricerche, Monterotondo, Italy

Disease and phenotype information

MGI phenotypes (allele matching)
  • abnormal cerebellar granule layer morphology / MGI
  • abnormal trigeminal nerve morphology / MGI
  • abnormal lipid homeostasis / MGI
  • abnormal hippocampal fimbria morphology / MGI
  • increased hepatocellular carcinoma incidence / MGI
  • abnormal myelin sheath morphology / MGI
  • increased liver tumor incidence / MGI
  • abnormal brain white matter morphology / MGI
  • integument phenotype / MGI

Literature references

  • Adult ceramide synthase 2 (CERS2)-deficient mice exhibit myelin sheath defects, cerebellar degeneration, and hepatocarcinomas.;Imgrund Silke, Hartmann Dieter, Farwanah Hany, Eckhardt Matthias, Sandhoff Roger, Degen Joachim, Gieselmann Volkmar, Sandhoff Konrad, Willecke Klaus, ;2009;The Journal of biological chemistry;284;33549-60; 19801672

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Order

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

  • Frozen embryos. Delivered in 4 weeks (after paperwork in place). €1740*
  • Rederivation of mice from frozen stock, delivery time available upon request . €3880*

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
For this strain no provider MTA is needed. Distribution is based on the EMMA conditions only.

EMMA conditions
Legally binding conditions for the transfer

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