STOCK Tg(LYZ-rtTA2S*M2,tetO-ELAVL1)632Dkon/Flmg

Status	Available to order
EMMA ID	EM:04971
Citation information	RRID:IMSR_EM:04971 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information.
International strain name	STOCK Tg(LYZ-rtTA2S*M2,tetO-ELAVL1)632Dkon/Flmg
Alternative name	TgLrT-HAHuRL.632
Strain type	Transgenic Strains
Allele/Transgene symbol	Tg(LYZ-rtTA2S*M2,tetO-ELAVL1)632Dkon
Gene/Transgene symbol	Tg(LYZ-rtTA2S*M2,tetO-ELAVL1)632Dkon

Information from provider

Provider	Dimitris KONTOYIANNIS
Provider affiliation	Immunology, B.S.R.C. Alexander Fleming
Genetic information	The complete cDNA sequence for human ELAVL1 (HuR) was obtained from the IMAGE clone number 2901220 (GenBank accession number BC003376) and was subcloned in-frame to an HA-epitope tag for the expression of an N-terminally tagged form. Subsequently, the HA-HuR cDNA was used for the generation of a transgenic construct that contained, in a 5' to 3' orientation, seven copies of the 42 bp tet operator sequence upstream of the minimal CMV promoter (Clontech); a globin intronic sequence for optimal transgene expression; the HA-HuR cDNA; and a bovine growth hormone polyadenylation signal. For the production of transgenic mice, fertilized (CBA x C57BL/6)F2 hybrid zygotes were coinjected with the transgenic devices using standard procedures.
Phenotypic information	Macrophage-restricted, Dox-inducible expression, not on polymorphonuclear leukocytes (PMNs) - high expression.
References	HuR as a negative posttranscriptional modulator in inflammation.;Katsanou Vicky, Papadaki Olympia, Milatos Stavros, Blackshear Perry J, Anderson Paul, Kollias George, Kontoyiannis Dimitris L, ;2005;Molecular cell;19;777-89; 16168373
Homozygous fertile	yes
Homozygous viable	yes
Homozygous matings required	no
Immunocompromised	no

Information from EMMA

Archiving centre	B.S.R.C. Alexander Fleming, Vari, Greece
Animals used for archiving	heterozygous CBA x C57BL/6 males, wild-type (CBA x C57BL/6)F1 females
Stage of embryos	2-cell

Literature references

HuR as a negative posttranscriptional modulator in inflammation.;Katsanou Vicky, Papadaki Olympia, Milatos Stavros, Blackshear Perry J, Anderson Paul, Kollias George, Kontoyiannis Dimitris L, ;2005;Molecular cell;19;777-89; 16168373

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Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

Frozen embryos. Delivered in 4 weeks (after paperwork in place). €1740^*
Frozen sperm. Delivered in 4 weeks (after paperwork in place). €1740^*
Rederivation of mice from frozen stock, delivery time available upon request . €3880^*

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

^* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

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Practical information

Example health report
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Material Transfer Agreement (MTA)
Distribution of this strain is subject to a provider MTA. Both signing of the MTA and submission of the online EMMA Mutant Request Form are required before material can be shipped.

EMMA conditions
Legally binding conditions for the transfer

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