C57BL/6NTac-Prmt1^{tm1a(EUCOMM)Wtsi}/H

Status	Available to order
EMMA ID	EM:05070
Citation information	RRID:IMSR_EM:05070 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information.
International strain name	C57BL/6NTac-Prmt1^{tm1a(EUCOMM)Wtsi}/H
Alternative name	EPD0070_1_B02
Strain type	Targeted Mutant Strains
Allele/Transgene symbol	Prmt1^{tm1a(EUCOMM)Wtsi}
Gene/Transgene symbol	Prmt1
Disclaimer	Please note that for EUCOMM and KOMP-CSD mice supplied to the scientific community by INFRAFRONTIER/EMMA: We can not guarantee a null mutation for Knock-out first alleles (tm1a alleles, see http://www.mousephenotype.org/about-ikmc/targeting-strategies) as the critical exon has not been deleted. That the structure of the targeted mutation in the ES cells obtained from EUCOMM/KOMP to generate EUCOMM/KOMP mice is not verified by INFRAFRONTIER/EMMA. It is recommended that the recipient confirms the mutation structure. No check for determining the copy number of the targeting construct in ES cells obtained from EUCOMM/KOMP is done by INFRAFRONTIER/EMMA. The level of quality control before mice are released is to confirm the individual mouse genotype by short range PCR.

Information from provider

Provider	MRC, Medical Research Council
Provider affiliation	Mary Lyon Centre at MRC Harwell
Genetic information	This mouse line originates from EUCOMM ES clone EPD0070_1_B02. For further details on the construction of this clone see the page at the IMPC portal.
Phenotypic information	Potential phenotyping data in the IMPC portal
References	None available

Information from EMMA

Archiving centre	Mary Lyon Centre at MRC Harwell, Oxford, United Kingdom
Animals used for archiving	heterozygous C57BL/6NTac males
Breeding at archiving centre	Chimeras were mated to C57BL/6N Tac USA and maintained on this background thereafter.

Disease and phenotype information

IMPC phenotypes (allele matching)

limb grasping / IMPC
increased circulating alkaline phosphatase level / IMPC
decreased circulating glycerol level / IMPC
decreased circulating total protein level / IMPC
preweaning lethality, complete penetrance / IMPC

IMPC phenotypes (gene matching)

increased circulating alkaline phosphatase level / IMPC
preweaning lethality, complete penetrance / IMPC
decreased circulating glycerol level / IMPC
limb grasping / IMPC
decreased circulating total protein level / IMPC

MGI phenotypes (allele matching)

increased circulating creatinine level / MGI

MGI phenotypes (gene matching)

absent amniotic folds / MGI
embryonic growth arrest / MGI
no phenotypic analysis / MGI
abnormal egg cylinder morphology / MGI
increased circulating creatinine level / MGI
empty decidua capsularis / MGI
embryonic lethality between implantation and somite formation, complete penetrance / MGI
absent proamniotic cavity / MGI
absent ectoplacental cavity / MGI

Information on how we integrate external resources can be found here

Order

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

Frozen sperm. Delivered in 4 weeks (after paperwork in place). €1740^*
Rederivation of mice from frozen stock, delivery time available upon request . €3880^*
LacZ tagged null allele (tm1b allele), service fee and delivery time available upon request
Tissue - Types of tissue, service fee and delivery time available upon request

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

^* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Genotyping protocol

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
MTA will be issued after an order has been submitted.

EMMA conditions
Legally binding conditions for the transfer

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C57BL/6NTac-Prmt1tm1a(EUCOMM)Wtsi/H