C57BL/6N-Cd28tm1a(EUCOMM)Hmgu/H

Status

Available to order

EMMA IDEM:05072
Citation informationRRID:IMSR_EM:05072 

Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information.

 
International strain nameC57BL/6N-Cd28tm1a(EUCOMM)Hmgu/H
Alternative nameHEPD0554_1_B05
Strain typeTargeted Mutant Strains
Allele/Transgene symbolCd28tm1a(EUCOMM)Hmgu
Gene/Transgene symbolCd28
DisclaimerPlease note that for EUCOMM and KOMP-CSD mice supplied to the scientific community by INFRAFRONTIER/EMMA:
  1. We can not guarantee a null mutation for Knock-out first alleles (tm1a alleles, see http://www.mousephenotype.org/about-ikmc/targeting-strategies) as the critical exon has not been deleted.
  2. That the structure of the targeted mutation in the ES cells obtained from EUCOMM/KOMP to generate EUCOMM/KOMP mice is not verified by INFRAFRONTIER/EMMA. It is recommended that the recipient confirms the mutation structure.
  3. No check for determining the copy number of the targeting construct in ES cells obtained from EUCOMM/KOMP is done by INFRAFRONTIER/EMMA.
  4. The level of quality control before mice are released is to confirm the individual mouse genotype by short range PCR.

Information from provider

Provider MRC, Medical Research Council
Provider affiliationMary Lyon Centre at MRC Harwell
Genetic informationThis mouse line originates from EUCOMM ES clone HEPD0554_1_B05. For further details on the construction of this clone see the page at the IMPC portal.
Phenotypic informationPotential phenotyping data in the IMPC portal
ReferencesNone available

Information from EMMA

Archiving centreMary Lyon Centre at MRC Harwell, Oxford, United Kingdom
Animals used for archivingheterozygous C57BL/6NTac males
Breeding at archiving centreChimeras were mated to C57BL/6N Tac USA and maintained on this background thereafter.

Disease and phenotype information

IMPC phenotypes (allele matching)
  • increased leukocyte cell number / IMPC
  • decreased T cell number / IMPC
  • increased CD4-positive, alpha-beta T cell number / IMPC
IMPC phenotypes (gene matching)
  • decreased T cell number / IMPC
  • increased leukocyte cell number / IMPC
  • increased CD4-positive, alpha-beta T cell number / IMPC
MGI phenotypes (gene matching)
  • enlarged thymus / MGI
  • decreased IgM level / MGI
  • brain inflammation / MGI
  • no abnormal phenotype detected / MGI
  • abnormal lymphopoiesis / MGI
  • decreased immunoglobulin level / MGI
  • abnormal immunoglobulin level / MGI
  • abnormal cytokine secretion / MGI
  • decreased susceptibility to experimental autoimmune encephalomyelitis / MGI
  • increased susceptibility to autoimmune diabetes / MGI
  • abnormal class switch recombination / MGI
  • abnormal regulatory T cell physiology / MGI
  • decreased regulatory T cell number / MGI
  • abnormal cytotoxic T cell physiology / MGI
  • decreased T cell proliferation / MGI
  • increased T cell proliferation / MGI
  • abnormal CD4-positive, alpha-beta T cell physiology / MGI
  • abnormal T-helper 1 physiology / MGI
  • abnormal T-helper 2 physiology / MGI
  • increased susceptibility to type I hypersensitivity reaction / MGI
  • decreased susceptibility to type IV hypersensitivity reaction / MGI
  • abnormal response to transplant / MGI
  • increased T cell apoptosis / MGI
  • decreased CD4-positive, alpha beta T cell number / MGI
  • abnormal germinal center B cell physiology / MGI
  • decreased spleen germinal center number / MGI
  • decreased IgG1 level / MGI
  • decreased IgG2a level / MGI
  • decreased IgG2b level / MGI
  • increased IgG2a level / MGI
  • increased interferon-gamma secretion / MGI
  • decreased interferon-gamma secretion / MGI
  • decreased circulating interferon-gamma level / MGI
  • decreased circulating interleukin-4 level / MGI
  • decreased interleukin-12 secretion / MGI
  • decreased interleukin-2 secretion / MGI
  • decreased interleukin-4 secretion / MGI
  • increased splenocyte proliferation / MGI
  • abnormal NK cell physiology / MGI

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