C57BL/6NTac-Afmidtm1a(EUCOMM)Wtsi/H

Status

Available to order

EMMA IDEM:05099
Citation informationRRID:IMSR_EM:05099 

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International strain nameC57BL/6NTac-Afmidtm1a(EUCOMM)Wtsi/H
Alternative nameEPD0155_6_A07
Strain typeTargeted Mutant Strains
Allele/Transgene symbolAfmidtm1a(EUCOMM)Wtsi
Gene/Transgene symbolAfmid
DisclaimerPlease note that for EUCOMM and KOMP-CSD mice supplied to the scientific community by INFRAFRONTIER/EMMA:
  1. We can not guarantee a null mutation for Knock-out first alleles (tm1a alleles, see http://www.mousephenotype.org/about-ikmc/targeting-strategies) as the critical exon has not been deleted.
  2. That the structure of the targeted mutation in the ES cells obtained from EUCOMM/KOMP to generate EUCOMM/KOMP mice is not verified by INFRAFRONTIER/EMMA. It is recommended that the recipient confirms the mutation structure.
  3. No check for determining the copy number of the targeting construct in ES cells obtained from EUCOMM/KOMP is done by INFRAFRONTIER/EMMA.
  4. The level of quality control before mice are released is to confirm the individual mouse genotype by short range PCR.

Information from provider

Provider MRC, Medical Research Council
Provider affiliationMary Lyon Centre at MRC Harwell
Genetic informationThis mouse line originates from EUCOMM ES clone EPD0155_6_A07. For further details on the construction of this clone see the page at the IMPC portal.
Phenotypic informationPotential phenotyping data in the IMPC portal
References
  • Loss of arylformamidase with reduced thymidine kinase expression leads to impaired glucose tolerance.;Hugill Alison J, Stewart Michelle E, Yon Marianne A, Probert Fay, Cox I Jane, Hough Tertius A, Scudamore Cheryl L, Bentley Liz, Wall Gary, Wells Sara E, Cox Roger D, ;2015;Biology open;4;1367-75; 26432886

Information from EMMA

Archiving centreMary Lyon Centre at MRC Harwell, Oxford, United Kingdom
Animals used for archivingheterozygous C57BL/6NTac males
Breeding at archiving centreChimeras were mated to C57BL/6N Tac USA and maintained on this background thereafter.

Disease and phenotype information

IMPC phenotypes (gene matching)
  • decreased grip strength / IMPC
  • increased circulating glucose level / IMPC
  • increased mean corpuscular volume / IMPC
  • increased mean corpuscular hemoglobin / IMPC
MGI phenotypes (allele matching)
  • polydipsia / MGI
  • hyperglycemia / MGI
  • polyuria / MGI
MGI phenotypes (gene matching)
  • hyperactivity / MGI
  • polydipsia / MGI
  • hyperglycemia / MGI
  • polyuria / MGI
  • decreased insulin secretion / MGI
  • abnormal pancreatic islet morphology / MGI
  • impaired glucose tolerance / MGI
  • renal/urinary system phenotype / MGI
  • liver/biliary system phenotype / MGI
  • growth/size/body region phenotype / MGI
  • increased circulating glucose level / MGI
  • decreased pancreatic islet number / MGI
  • abnormal urine homeostasis / MGI
  • increased circulating fructosamine level / MGI

Literature references

  • Loss of arylformamidase with reduced thymidine kinase expression leads to impaired glucose tolerance.;Hugill Alison J, Stewart Michelle E, Yon Marianne A, Probert Fay, Cox I Jane, Hough Tertius A, Scudamore Cheryl L, Bentley Liz, Wall Gary, Wells Sara E, Cox Roger D, ;2015;Biology open;4;1367-75; 26432886

Information on how we integrate external resources can be found here

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Practical information

Genotyping protocol

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