FVB-Tg(Fabp2-Arg1)1Wla/Cnbc

Status	Available to order
EMMA ID	EM:05159
Citation information	RRID:IMSR_EM:05159 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information.
International strain name	FVB-Tg(Fabp2-Arg1)1Wla/Cnbc
Alternative name	FA/2 FVB
Strain type	Transgenic Strains
Allele/Transgene symbol	Tg(Fabp2-Arg1)1Wla
Gene/Transgene symbol	Tg(Fabp2-Arg1)1Wla

Information from provider

Provider	Wouter Lamers
Provider affiliation	Maastricht University
Genetic information	Transgenic mouse line that overexpresses arginase I in the enterocytes only (cDNA of rat arginase 1 under control of FABPi promoter).
Phenotypic information	The phenotype of suckling F/A2 mice (hypoargininemia, reduced hair and muscle growth, impaired B-cell maturation, impaired pituitary growth-hormone secretion, and decreased liver Igf1 mRNA production and plasma IGF1 concentration) results from activation of the general control non-derepressible 2 (GCN2) stress kinase signaling pathway, without impacting mTORC1-mediated signaling.
Breeding history	Backcrossed more than 10 times to C57BL/6J, bred as heterozygote versus wild type.
References	Effect of arginine deficiency on arginine-dependent post-translational protein modifications in mice.;Kwikkers Karin L, Ruijter Jan M, Labruyère Wil T, McMahon Kathryn K, Lamers Wouter H, ;2005;The British journal of nutrition;93;183-9; 15788111 Arginine deficiency affects early B cell maturation and lymphoid organ development in transgenic mice.;de Jonge Wouter J, Kwikkers Karin L, te Velde Anje A, van Deventer Sander J H, Nolte Martijn A, Mebius Reina E, Ruijter Jan M, Lamers Marinus C, Lamers Wouter H, ;2002;The Journal of clinical investigation;110;1539-48; 12438451 Overexpression of arginase I in enterocytes of transgenic mice elicits a selective arginine deficiency and affects skin, muscle, and lymphoid development.;de Jonge Wouter J, Hallemeesch Marcella M, Kwikkers Karin L, Ruijter Jan M, de Gier-de Vries Corrie, van Roon Marian A, Meijer Alfred J, Marescau Bart, de Deyn Peter P, Deutz Nicolaas E P, Lamers Wouter H, ;2002;The American journal of clinical nutrition;76;128-40; 12081826 Overexpression of arginase alters circulating and tissue amino acids and guanidino compounds and affects neuromotor behavior in mice.;de Jonge W J, Marescau B, D'Hooge R, De Deyn P P, Hallemeesch M M, Deutz N E, Ruijter J M, Lamers W H, ;2001;The Journal of nutrition;131;2732-40; 11584097 Arginine deficiency causes runting in the suckling period by selectively activating the stress kinase GCN2.;Marion Vincent, Sankaranarayanan Selvakumari, de Theije Chiel, van Dijk Paul, Lindsey Patrick, Lamers Marinus C, Harding Heather P, Ron David, Lamers Wouter H, Köhler S Eleonore, ;2011;The Journal of biological chemistry;286;8866-74; 21239484
Homozygous fertile	no
Homozygous viable	yes
Homozygous matings required	no
Immunocompromised	no

Information from EMMA

Archiving centre	CNB-CSIC, Centro Nacional de Biotecnologia, Madrid, Spain
Animals used for archiving	heterozygous FVB/N males, wild-type FVB/N females
Stage of embryos	8-cell

Disease and phenotype information

MGI phenotypes (allele matching)

abnormal hair follicle morphology / MGI
distorted hair follicle pattern / MGI
sparse hair / MGI
delayed hair appearance / MGI
small spleen / MGI
abnormal Peyer's patch morphology / MGI
abnormal epidermal layer morphology / MGI
hyperkeratosis / MGI
decreased body weight / MGI
hyperactivity / MGI
postnatal growth retardation / MGI
abnormal homeostasis / MGI
decreased IgM level / MGI
abnormal B cell differentiation / MGI
decreased spleen weight / MGI
decreased B cell number / MGI
abnormal amino acid level / MGI
abnormal enzyme/coenzyme activity / MGI
decreased Peyer's patch number / MGI
small Peyer's patches / MGI
decreased transitional stage B cell number / MGI
decreased pre-B cell number / MGI
abnormal spleen B cell follicle morphology / MGI
abnormal enterocyte physiology / MGI
decreased skeletal muscle fiber diameter / MGI
abnormal blood homeostasis / MGI

MGI phenotypes (gene matching)

abnormal hair follicle morphology / MGI
distorted hair follicle pattern / MGI
sparse hair / MGI
delayed hair appearance / MGI
small spleen / MGI
abnormal Peyer's patch morphology / MGI
abnormal epidermal layer morphology / MGI
hyperkeratosis / MGI
decreased body weight / MGI
hyperactivity / MGI
postnatal growth retardation / MGI
decreased IgM level / MGI
abnormal B cell differentiation / MGI
decreased spleen weight / MGI
decreased B cell number / MGI
abnormal amino acid level / MGI
abnormal enzyme/coenzyme activity / MGI
decreased Peyer's patch number / MGI
small Peyer's patches / MGI
decreased transitional stage B cell number / MGI
decreased pre-B cell number / MGI
abnormal spleen B cell follicle morphology / MGI
abnormal enterocyte physiology / MGI
decreased skeletal muscle fiber diameter / MGI
increased ornithine level / MGI
increased circulating glycine level / MGI
decreased circulating arginine level / MGI
decreased arginine level / MGI

Literature references

Effect of arginine deficiency on arginine-dependent post-translational protein modifications in mice.;Kwikkers Karin L, Ruijter Jan M, Labruyère Wil T, McMahon Kathryn K, Lamers Wouter H, ;2005;The British journal of nutrition;93;183-9; 15788111
Arginine deficiency affects early B cell maturation and lymphoid organ development in transgenic mice.;de Jonge Wouter J, Kwikkers Karin L, te Velde Anje A, van Deventer Sander J H, Nolte Martijn A, Mebius Reina E, Ruijter Jan M, Lamers Marinus C, Lamers Wouter H, ;2002;The Journal of clinical investigation;110;1539-48; 12438451
Overexpression of arginase I in enterocytes of transgenic mice elicits a selective arginine deficiency and affects skin, muscle, and lymphoid development.;de Jonge Wouter J, Hallemeesch Marcella M, Kwikkers Karin L, Ruijter Jan M, de Gier-de Vries Corrie, van Roon Marian A, Meijer Alfred J, Marescau Bart, de Deyn Peter P, Deutz Nicolaas E P, Lamers Wouter H, ;2002;The American journal of clinical nutrition;76;128-40; 12081826
Overexpression of arginase alters circulating and tissue amino acids and guanidino compounds and affects neuromotor behavior in mice.;de Jonge W J, Marescau B, D'Hooge R, De Deyn P P, Hallemeesch M M, Deutz N E, Ruijter J M, Lamers W H, ;2001;The Journal of nutrition;131;2732-40; 11584097
Arginine deficiency causes runting in the suckling period by selectively activating the stress kinase GCN2.;Marion Vincent, Sankaranarayanan Selvakumari, de Theije Chiel, van Dijk Paul, Lindsey Patrick, Lamers Marinus C, Harding Heather P, Ron David, Lamers Wouter H, Köhler S Eleonore, ;2011;The Journal of biological chemistry;286;8866-74; 21239484

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Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

Frozen embryos. Delivered in 4 weeks (after paperwork in place). €1740^*
Frozen sperm. Delivered in 4 weeks (after paperwork in place). €1740^*
Rederivation of mice from frozen stock, delivery time available upon request . €3880^*

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Practical information

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