STOCK Tg(Gfap-Pten*)6Rpm/Cnbc

Status	Available to order
EMMA ID	EM:05237
Citation information	RRID:IMSR_EM:05237 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information.
International strain name	STOCK Tg(Gfap-Pten*)6Rpm/Cnbc
Alternative name	GL1 GFAP-Pten qma
Strain type	Transgenic Strains
Allele/Transgene symbol	Tg(Gfap-PTEN*)6Rpm
Gene/Transgene symbol	Tg(Gfap-PTEN*)6Rpm

Information from provider

Provider	RAFAEL PULIDO-MURILLO
Provider affiliation	LABORATORY OF MOLECULAR BIOLOGY OF CANCER, CENTRO DE INVESTIGACIÓN PRINCIPE FELIPE
Genetic information	Transgenic mouse in strain C57BL/6 x SJL; gene affected Pten (MGI ref. 109583); Pten is mutated in residues phosphorylated by CK2 (qma, Ser370Ala, Ser380Ala, Thr382Ala, Thr383Ala, and Ser385Ala). The subcellular localization of PTEN qma is nuclear in cell lines and favours the apoptosis.
Phenotypic information	The expression of the transgene does not have any phenotype in the mouse strain.
Breeding history	The founder was crossed with the wt strain C57BL/6 x SJL to generate the heterozygous animals. The heterozygous animals were backcrossed 6 times with brothers and sisters until we obtained the homozygous animals.
References	Nuclear localization of PTEN by a Ran-dependent mechanism enhances apoptosis: Involvement of an N-terminal nuclear localization domain and multiple nuclear exclusion motifs.;Gil Anabel, Andrés-Pons Amparo, Fernández Elena, Valiente Miguel, Torres Josema, Cervera Javier, Pulido Rafael, ;2006;Molecular biology of the cell;17;4002-13; 16807353 Nuclear PTEN: a tale of many tails.;Gil A, Andrés-Pons A, Pulido R, ;2007;Cell death and differentiation;14;395-9; 17186024
Homozygous fertile	yes
Homozygous viable	yes
Homozygous matings required	yes
Immunocompromised	no

Information from EMMA

Archiving centre	CNB-CSIC, Centro Nacional de Biotecnologia, Madrid, Spain
Animals used for archiving	homozygous C57BL/6 x SJL males, homozygous C57BL/6 x SJL females
Stage of embryos	2-cell

Literature references

Nuclear localization of PTEN by a Ran-dependent mechanism enhances apoptosis: Involvement of an N-terminal nuclear localization domain and multiple nuclear exclusion motifs.;Gil Anabel, Andrés-Pons Amparo, Fernández Elena, Valiente Miguel, Torres Josema, Cervera Javier, Pulido Rafael, ;2006;Molecular biology of the cell;17;4002-13; 16807353
Nuclear PTEN: a tale of many tails.;Gil A, Andrés-Pons A, Pulido R, ;2007;Cell death and differentiation;14;395-9; 17186024

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Order

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

Frozen embryos. Delivered in 4 weeks (after paperwork in place). €1740^*
Frozen sperm. Delivered in 4 weeks (after paperwork in place). €1740^*
Rederivation of mice from frozen stock, delivery time available upon request . €3880^*

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

^* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Genotyping protocol

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
For this strain no provider MTA is needed. Distribution is based on the EMMA conditions only.

EMMA conditions
Legally binding conditions for the transfer

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