B6N.129P2(Cg)-Mir451atm1.1Doca/Cnrm
| Status | Available to order |
| EMMA ID | EM:05329 |
| International strain name | B6N.129P2(Cg)-Mir451atm1.1Doca/Cnrm |
| Alternative name | Mir451 |
| Strain type | Targeted Mutant Strains : Conditional mutation |
| Allele/Transgene symbol | Mir451atm1.1Doca, |
| Gene/Transgene symbol | Mir451a |
Information from provider
| Provider | Dónal O |
| Provider affiliation | Mouse Biology Unit, European Molecular Biology Laboratory |
| Genetic information | An FRT flanked neo cassette with a 5' loxP site was inserted downstream of the miR-144/451 locus. An additional loxP site was inserted between the two microRNAs. Flp mediated recombination removed the neo cassette leaving miR-451 floxed. The miR-451 targeting strategy allows cre-mediated deletion of miR-451 within the miR-144/451 cluster, leaving expression of miR-144 intact. |
| Phenotypic information | Mice deficient for the miR-451 display a cell autonomous impairment of late erythroblast maturation, resulting in erythroid hyperplasia, splenomegaly, and a mild anemia. |
| Breeding history | Upon germ line transmission, the mice carrying the targeted allele were crossed to FLP-expressing transgenic mice (FLPeR) to remove the frt flanked neo-resistance cassette. The resulting mice were backcrossed to C57BL/6N to remove the FLP transgene. The offspring carrying the loxP-flanked allele were backcrossed 6 generations to C57BL/6N. |
| References |
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| Homozygous fertile | yes |
| Homozygous viable | yes |
| Homozygous matings required | no |
| Immunocompromised | no |
Information from EMMA
| Archiving centre | CNR, Consiglio Nazionale delle Ricerche, Monterotondo, Italy |
Literature references
- The miR-144/451 locus is required for erythroid homeostasis.;Rasmussen Kasper D, Simmini Salvatore, Abreu-Goodger Cei, Bartonicek Nenad, Di Giacomo Monica, Bilbao-Cortes Daniel, Horos Rastislav, Von Lindern Marieke, Enright Anton J, O'Carroll Dónal, ;2010;The Journal of experimental medicine;207;1351-8; 20513743