MGI phenotypes (allele matching)
B6N.Cg-Mirc12tm1.1Doca/Cnrm
| Status | Available to order |
| EMMA ID | EM:05331 |
| International strain name | B6N.Cg-Mirc12tm1.1Doca/Cnrm |
| Alternative name | MiR-144/451 |
| Strain type | Targeted Mutant Strains : Knock-in |
| Allele/Transgene symbol | Mirc12tm1.1Doca, |
| Gene/Transgene symbol | Mirc12 |
Information from provider
| Provider | Dónal O |
| Provider affiliation | Mouse Biology Unit, European Molecular Biology Laboratory |
| Genetic information | An enhanced GFP (eGFP) expression cassette with a 3'- FRT flanked neor cassette was inserted into the miR-144/451 locus. Flp mediated recombination removed the neor cassette. The presence of the eGFP cassette disrupts expression of miR-144 and miR-451 and allows tracing of transcriptional activity from the miR-144/451 locus. |
| Phenotypic information | Homozygous mice carrying the miR-144/451-EGFP-KI allele display an impairment of late erythroblast maturation, resulting in erythroid hyperplasia, splenomegaly, and a mild anemia. |
| Breeding history | Upon germ line transmission, the mice carrying the targeted allele were crossed to FLP-expressing transgenic mice (FLPeR) to remove the frt flanked neor cassette. The resulting mice were then backcrossed to C57BL/6N to remove the FLP transgene. The offspring carrying the mutation were backcrossed 5 generations to C57BL/6N. |
| References |
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| Homozygous fertile | yes |
| Homozygous viable | yes |
| Homozygous matings required | no |
| Immunocompromised | no |
Information from EMMA
| Archiving centre | CNR, Consiglio Nazionale delle Ricerche, Monterotondo, Italy |
Disease and phenotype information
Literature references
- The miR-144/451 locus is required for erythroid homeostasis.;Rasmussen Kasper D, Simmini Salvatore, Abreu-Goodger Cei, Bartonicek Nenad, Di Giacomo Monica, Bilbao-Cortes Daniel, Horos Rastislav, Von Lindern Marieke, Enright Anton J, O'Carroll Dónal, ;2010;The Journal of experimental medicine;207;1351-8; 20513743