MGI phenotypes (allele matching)
B6N.Cg-Piwil2tm1.1Doca/Cnrm
| Status | Available to order |
| EMMA ID | EM:05451 |
| International strain name | B6N.Cg-Piwil2tm1.1Doca/Cnrm |
| Alternative name | Mili DAH |
| Strain type | Targeted Mutant Strains : Knock-in |
| Allele/Transgene symbol | Piwil2tm1.1Doca, |
| Gene/Transgene symbol | Piwil2 |
Information from provider
| Provider | Donal O'Carroll |
| Provider affiliation | EMBL Mouse Biology Unit |
| Genetic information | To generate MiliD813A allele the wild type exon 21 was replaced with a mutant exon where the aspartic acid 813 codon is mutated to encode an alanine. A targeting construct was recombineered that contains homology arms, an frt flanked neomycin (neo) cassette 3' of exon 21 that contains the MiliD813A mutation. Flp-mediated recombination causes an excision of the Neor-frt flanked cassette that results in the MiliDAH allele. |
| Phenotypic information | Homozygous male mice for Mili DAH allele are viable and healthy but present an impairment of spermatogenesis that results in testis atrophy and infertility. The mutation does not affect female fertility at all. |
| Breeding history | Mice carrying the targeted allele were crossed with Flp expressing transgenic mice (FlpeR) to remove the Frt-flanked Neor cassette. The resulting offspring was backcrossed to C57BL/6N to remove the FLP transgene. Mice carrying the mutation were then backcrossed 7 generations to C57BL/6N. |
| References |
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| Homozygous fertile | not known |
| Homozygous viable | not known |
| Homozygous matings required | no |
| Immunocompromised | no |
Information from EMMA
| Archiving centre | CNR, Consiglio Nazionale delle Ricerche, Monterotondo, Italy |
Disease and phenotype information
Literature references
- The endonuclease activity of Mili fuels piRNA amplification that silences LINE1 elements.;De Fazio Serena, Bartonicek Nenad, Di Giacomo Monica, Abreu-Goodger Cei, Sankar Aditya, Funaya Charlotta, Antony Claude, Moreira Pedro N, Enright Anton J, O'Carroll Dónal, ;2011;Nature;480;259-63; 22020280