MGI phenotypes (allele matching)
B6N.Cg-Piwil4tm1.1Doca/Cnrm
| Status | Available to order |
| EMMA ID | EM:05453 |
| International strain name | B6N.Cg-Piwil4tm1.1Doca/Cnrm |
| Alternative name | Miwi2 DAH |
| Strain type | Targeted Mutant Strains : Knock-in |
| Allele/Transgene symbol | Piwil4tm1.1Doca, |
| Gene/Transgene symbol | Piwil4 |
Information from provider
| Provider | Donal O'Carroll |
| Provider affiliation | EMBL Mouse Biology Unit |
| Genetic information | To generate Miwi2 D761A allele the wild-type exon 17 was replaced with a mutant exon where the aspartic acid 761 codon is mutated to encode an alanine. A targeting construct was recombineered that contains homology arms, an frt flanked neomycin (neo) cassette 3' of exon 17 that contains the Miwi2 D761A mutation; flp-mediated recombination caused an excision of the Neor-frt flanked cassette that results in the Miwi2 DAH allele. |
| Phenotypic information | Homozygous male and female mice for Miwi2 DAH allele are viable, healthy and fertile. Miwi2 DAH mice do not present any apparent abnormalities. |
| Breeding history | Mice carrying the targeted allele were crossed with flp-expressing transgenic mice (FlpeR) to remove the Frt-flanked Neor cassette. The resulting offspring was backcrossed to C57BL/6N to remove the FLP transgene. Mice carrying the mutation were then backcrossed 8 generations to C57BL/6N. |
| References |
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| Homozygous fertile | yes |
| Homozygous viable | yes |
| Homozygous matings required | no |
| Immunocompromised | no |
Information from EMMA
| Archiving centre | CNR, Consiglio Nazionale delle Ricerche, Monterotondo, Italy |
Disease and phenotype information
Literature references
- The endonuclease activity of Mili fuels piRNA amplification that silences LINE1 elements.;De Fazio Serena, Bartonicek Nenad, Di Giacomo Monica, Abreu-Goodger Cei, Sankar Aditya, Funaya Charlotta, Antony Claude, Moreira Pedro N, Enright Anton J, O'Carroll Dónal, ;2011;Nature;480;259-63; 22020280