(C57BL/6J x B10.Cg-H2k Tg(Cd74-rtTA)#Doi Tg(tetO-Cd74/MCC)#Doi)F1/Ieg
| Status | Available to order |
| EMMA ID | EM:05574 |
| Citation information | RRID:IMSR_EM:05574 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information. |
| International strain name | (C57BL/6J x B10.Cg-H2k Tg(Cd74-rtTA)#Doi Tg(tetO-Cd74/MCC)#Doi)F1/Ieg |
| Alternative name | Ii-rTAxTIM |
| Strain type | Transgenic Strains |
| Allele/Transgene symbol | Tg(Cd74-rtTA)#Doi, Tg(tetO-Cd74/MCC)#Doi |
| Gene/Transgene symbol | Tg(Cd74-rtTA)#Doi, Tg(tetO-Cd74/MCC)#Doi |
Information from provider
| Provider | Reinhard Obst |
| Provider affiliation | Institute for Immunology, Ludwig-Maximilians-Universität Munich |
| Additional owner | Christophe Benoist, Department of Microbiology and Immunology, Harvard Medical School, Boston MA, USA |
| Genetic information | Tg(Cd74-rtTA)#Doi: The Cd74 promoter, together with the H2-Ea enhancer, drives the expression of an improved reverse tet transactivator, rtTA-S2. Four independent lines were produced. An un-named line showing the lowest liver expression was used. Tg(tetO-Cd74/MCC)#Doi: Seven tetO sequences were combined with the CMV minimal promoter to drive expression, when induced by doxycycline, of a construct composed of the Cd74 cDNA and a segment of moth cytocrome c cDNA encoding amino acid residues 93 to 103, which replace the CLIP region of Cd74 (amino acid residues 88 to 98). |
| Phenotypic information | Both transgenes confer inducibility of the moth cytochrome C antigen presented by H-2Ek following doxycyclin administration, mostly by dendritic cells. |
| Breeding history | Backcrossed more than 10 generations to B10.BR-H2k H2-T18a/SgSnJ. Currently brother x sister matings. C57BL/6 or C57BL/10 best conventional background usable for this line. |
| References |
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| Homozygous fertile | yes |
| Homozygous viable | yes |
| Homozygous matings required | no |
| Immunocompromised | no |
Information from EMMA
| Archiving centre | Helmholtz Zentrum Muenchen - German Research Center for Environmental Health (GmbH), Oberschleißheim, Germany |
| Animals used for archiving | heterozygous B10.BR-H2k males, wild-type C57BL/6J females |
| Stage of embryos | 2-cell |
Literature references
- Antigen persistence is required throughout the expansion phase of a CD4(+) T cell response.;Obst Reinhard, van Santen Hisse-Martien, Mathis Diane, Benoist Christophe, ;2005;The Journal of experimental medicine;201;1555-65; 15897273
- Number of T reg cells that differentiate does not increase upon encounter of agonist ligand on thymic epithelial cells.;van Santen Hisse-Martien, Benoist Christophe, Mathis Diane, ;2004;The Journal of experimental medicine;200;1221-30; 15534371