B6.Cg-Piwil1tm1.1Embrp/Cnrm

Status

Available to order

EMMA IDEM:05595
International strain nameB6.Cg-Piwil1tm1.1Embrp/Cnrm
Alternative nameMiwi Slicer mutant
Strain typeTargeted Mutant Strains : Knock-in
Allele/Transgene symbolPiwil1tm1.1Embrp,
Gene/Transgene symbolPiwil1

Information from provider

ProviderRamesh PILLAI
Provider affiliationMouse Biology Unit, European Molecular Biology Laboratory
Genetic informationPiwil1 (Miwi) knock-in allele. Carries a single amino acid mutation ADH (D633A). Inactivates the slicer (small RNA-guided RNase) activity.
Phenotypic informationOnly males show infertility. Block in spermatogenesis at elongating spermatids. Dominant negative.
References
  • Miwi catalysis is required for piRNA amplification-independent LINE1 transposon silencing.;Reuter Michael, Berninger Philipp, Chuma Shinichiro, Shah Hardik, Hosokawa Mihoko, Funaya Charlotta, Antony Claude, Sachidanandam Ravi, Pillai Ramesh S, ;2011;Nature;480;264-7; 22121019
Homozygous fertileno
Homozygous viableyes
Homozygous matings requiredno
Immunocompromisedno

Information from EMMA

Archiving centreCNR, Consiglio Nazionale delle Ricerche, Monterotondo, Italy

Disease and phenotype information

MGI phenotypes (allele matching)
  • male infertility / MGI
  • azoospermia / MGI
  • reproductive system phenotype / MGI
  • abnormal spermatid morphology / MGI
  • arrest of spermatogenesis / MGI

Literature references

  • Miwi catalysis is required for piRNA amplification-independent LINE1 transposon silencing.;Reuter Michael, Berninger Philipp, Chuma Shinichiro, Shah Hardik, Hosokawa Mihoko, Funaya Charlotta, Antony Claude, Sachidanandam Ravi, Pillai Ramesh S, ;2011;Nature;480;264-7; 22121019

Information on how we integrate external resources can be found here

Order

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

  • Frozen embryos. Delivered in 4 weeks (after paperwork in place). €1740*
  • Rederivation of mice from frozen stock, delivery time available upon request . €3880*

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
For this strain no provider MTA is needed. Distribution is based on the EMMA conditions only.

EMMA conditions
Legally binding conditions for the transfer

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