- decreased cardiac muscle contractility / IMPC
B6(Cg)-Clec9atm1.1Crs/H
Status | Available to order |
EMMA ID | EM:05605 |
Citation information | RRID:IMSR_EM:05605 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information. |
International strain name | B6(Cg)-Clec9atm1.1Crs/H |
Alternative name | DNGR-1 KO C57BL/6J |
Strain type | Targeted Mutant Strains : Knock-out |
Allele/Transgene symbol | Clec9atm1.1Crs |
Gene/Transgene symbol | Clec9a |
Information from provider
Provider | Neil Rogers |
Provider affiliation | Translational Oncology, Cancer Research UK |
Additional owner | Cancer Research UK (CRUK), London, UK |
Genetic information | Homozygous for knock-out mutation (a knock-in of a farnesylated form of EGFP inserted in frame with the two first amino acids of the gene locus). Half of the exon 1 and the entire exon 2 were eliminated and transcription following the EGFP is terminated by a strong polyadenylation signal. A loxP flanked neomycin cassette was included downstream of EGFP for targeting purposes and was subsequently deleted by crossing to a PGK-Cre mouse. |
Phenotypic information | The CD8+ve family of dendritic cells lack expression of DNGR-1 (CLEC9A) and are impaired in the cross-priming of T-cells. |
Breeding history | Knock-out generated in the (129S6 x C57BL/6)F1 derived ES cell line S6B6 (equivalent to B6 N1). After crossing 2x to C57BL/6J (Charles River), they were crossed 1x to C57BL/6J PGK-Cre to delete the NeoR gene. The strain is now Cre negative after being crossed a further 7x to C57BL/6J 7x to C57BL/6J Charles River. |
References |
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Homozygous fertile | yes |
Homozygous viable | yes |
Homozygous matings required | no |
Immunocompromised | no |
Information from EMMA
Archiving centre | Mary Lyon Centre at MRC Harwell, Oxford, United Kingdom |
Animals used for archiving | homozygous C57BL/6J males |
Breeding at archiving centre | Males were archived upon arrival. No breeding was performed at the archiving centre. |
Disease and phenotype information
IMPC phenotypes (gene matching)
MGI phenotypes (allele matching)
- abnormal dendritic cell antigen presentation / MGI
Literature references
- Identification of a dendritic cell receptor that couples sensing of necrosis to immunity.;Sancho David, Joffre Olivier P, Keller Anna M, Rogers Neil C, Martínez Dolores, Hernanz-Falcón Patricia, Rosewell Ian, Reis e Sousa Caetano, ;2009;Nature;458;899-903; 19219027
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