C57BL/6N-Mapkapk2^{tm1a(EUCOMM)Hmgu}/H

Status	Available to order
EMMA ID	EM:05658
Citation information	RRID:IMSR_EM:05658 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information.
International strain name	C57BL/6N-Mapkapk2^{tm1a(EUCOMM)Hmgu}/H
Alternative name	HEPD0672_2_A02
Strain type	Targeted Mutant Strains
Allele/Transgene symbol	Mapkapk2^{tm1a(EUCOMM)Hmgu}
Gene/Transgene symbol	Mapkapk2
Disclaimer	Please note that for EUCOMM and KOMP-CSD mice supplied to the scientific community by INFRAFRONTIER/EMMA: We can not guarantee a null mutation for Knock-out first alleles (tm1a alleles, see http://www.mousephenotype.org/about-ikmc/targeting-strategies) as the critical exon has not been deleted. That the structure of the targeted mutation in the ES cells obtained from EUCOMM/KOMP to generate EUCOMM/KOMP mice is not verified by INFRAFRONTIER/EMMA. It is recommended that the recipient confirms the mutation structure. No check for determining the copy number of the targeting construct in ES cells obtained from EUCOMM/KOMP is done by INFRAFRONTIER/EMMA. The level of quality control before mice are released is to confirm the individual mouse genotype by short range PCR.

Information from provider

Provider	MRC, Medical Research Council
Provider affiliation	Mary Lyon Centre at MRC Harwell
Genetic information	This mouse line originates from EUCOMM ES clone HEPD0672_2_A02. For further details on the construction of this clone see the page at the IMPC portal.
Phenotypic information	Potential phenotyping data in the IMPC portal
References	None available

Information from EMMA

Archiving centre	Mary Lyon Centre at MRC Harwell, Oxford, United Kingdom
Animals used for archiving	heterozygous C57BL/6NTac males
Breeding at archiving centre	Chimeras were crossed to C57BL/6N Tac USA and maintained on this background thereafter.

Disease and phenotype information

IMPC phenotypes (gene matching)

abnormal bone structure / IMPC
increased circulating glucose level / IMPC
abnormal optic disk morphology / IMPC
abnormal clavicle morphology / IMPC
abnormal joint morphology / IMPC
increased large unstained cell number / IMPC
increased eosinophil cell number / IMPC
increased leukocyte cell number / IMPC

MGI phenotypes (gene matching)

altered response to myocardial infarction / MGI
impaired macrophage phagocytosis / MGI
no abnormal phenotype detected / MGI
increased susceptibility to bacterial infection / MGI
abnormal cytokine secretion / MGI
decreased myocardial infarction size / MGI
decreased cardiomyocyte apoptosis / MGI
abnormal macrophage derived foam cell morphology / MGI
increased spleen germinal center size / MGI
decreased circulating tumor necrosis factor level / MGI
decreased tumor necrosis factor secretion / MGI
decreased interferon-gamma secretion / MGI
increased interleukin-1 beta secretion / MGI
abnormal cytokine level / MGI
decreased susceptibility to endotoxin shock / MGI
increased susceptibility to bacterial infection induced morbidity/mortality / MGI

Information on how we integrate external resources can be found here

Order

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

Frozen sperm. Delivered in 4 weeks (after paperwork in place). €1740^*
Rederivation of mice from frozen stock, delivery time available upon request . €3880^*
LacZ tagged null allele (tm1b allele), service fee and delivery time available upon request
Conditional ready allele (tm1c allele), service fee and delivery time available upon request
Reporterless null allele (tm1d allele), service fee and delivery time available upon request
Tissue - Types of tissue, service fee and delivery time available upon request

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

^* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Genotyping protocol

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
MTA will be issued after an order has been submitted.

EMMA conditions
Legally binding conditions for the transfer

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C57BL/6N-Mapkapk2tm1a(EUCOMM)Hmgu/H