C57BL/6NTac-Ehd1^{tm1a(EUCOMM)Wtsi}/WtsiBiat

Status	Available to order
EMMA ID	EM:05712
Citation information	RRID:IMSR_EM:05712 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information.
International strain name	C57BL/6NTac-Ehd1^{tm1a(EUCOMM)Wtsi}/WtsiBiat
Alternative name	EPD0114_1_D05
Strain type	Targeted Mutant Strains
Allele/Transgene symbol	Ehd1^{tm1a(EUCOMM)Wtsi}
Gene/Transgene symbol	Ehd1
Disclaimer	Please note that for EUCOMM and KOMP-CSD mice supplied to the scientific community by INFRAFRONTIER/EMMA: We can not guarantee a null mutation for Knock-out first alleles (tm1a alleles, see http://www.mousephenotype.org/about-ikmc/targeting-strategies) as the critical exon has not been deleted. That the structure of the targeted mutation in the ES cells obtained from EUCOMM/KOMP to generate EUCOMM/KOMP mice is not verified by INFRAFRONTIER/EMMA. It is recommended that the recipient confirms the mutation structure. No check for determining the copy number of the targeting construct in ES cells obtained from EUCOMM/KOMP is done by INFRAFRONTIER/EMMA. The level of quality control before mice are released is to confirm the individual mouse genotype by short range PCR.

Information from provider

Provider	Wellcome Trust Sanger Institute
Provider affiliation	Wellcome Trust Sanger Institute
Genetic information	This mouse line originates from EUCOMM ES clone EPD0114_1_D05. For further details on the construction of this clone see the page at the IMPC portal.
Phenotypic information	Potential phenotyping data in the IMPC portal
References	None available

Information from EMMA

Archiving centre	University of Veterinary Medicine, Vienna, Austria

Disease and phenotype information

IMPC phenotypes (allele matching)

increased circulating LDL cholesterol level / IMPC
increased circulating HDL cholesterol level / IMPC
increased circulating alanine transaminase level / IMPC
increased circulating chloride level / IMPC
abnormal eyelid aperture / IMPC
increased circulating cholesterol level / IMPC
process of degenerative change / IMPC
developmental dysplasia / IMPC

IMPC phenotypes (gene matching)

increased circulating HDL cholesterol level / IMPC
increased circulating cholesterol level / IMPC
increased circulating chloride level / IMPC
developmental dysplasia / IMPC
increased circulating LDL cholesterol level / IMPC
increased circulating alanine transaminase level / IMPC
abnormal eyelid aperture / IMPC
process of degenerative change / IMPC

MGI phenotypes (allele matching)

increased circulating chloride level / MGI
increased mean corpuscular hemoglobin / MGI
abnormal eyelid aperture / MGI

MGI phenotypes (gene matching)

malocclusion / MGI
seminiferous tubule degeneration / MGI
abnormal spermatogenesis / MGI
decreased body weight / MGI
decreased body size / MGI
anophthalmia / MGI
microphthalmia / MGI
cataract / MGI
male infertility / MGI
no abnormal phenotype detected / MGI
abnormal germ cell morphology / MGI
no phenotypic analysis / MGI
increased circulating chloride level / MGI
abnormal cell migration / MGI
abnormal eyelid aperture / MGI
abnormal spermiation / MGI
decreased testis weight / MGI
azoospermia / MGI
abnormal male meiosis / MGI
cellular phenotype / MGI
increased mean corpuscular hemoglobin / MGI
abnormal cell physiology / MGI
abnormal spermatogonia morphology / MGI
abnormal spermatocyte morphology / MGI
abnormal spermatid morphology / MGI
arrest of spermiogenesis / MGI
abnormal male germ cell apoptosis / MGI
abnormal acrosome morphology / MGI
abnormal Sertoli cell phagocytosis / MGI
postnatal lethality, incomplete penetrance / MGI
perinatal lethality, incomplete penetrance / MGI
decreased fibroblast cell migration / MGI

Information on how we integrate external resources can be found here

Order

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

Frozen embryos. Delivered in 4 weeks (after paperwork in place). €1740^*
Rederivation of mice from frozen stock, delivery time available upon request . €3880^*
LacZ tagged null allele (tm1b allele), service fee and delivery time available upon request
Conditional ready allele (tm1c allele), service fee and delivery time available upon request
Reporterless null allele (tm1d allele), service fee and delivery time available upon request

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

^* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Genotyping protocol

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
Distribution of this strain is subject to a provider MTA. Both signing of the MTA and submission of the online EMMA Mutant Request Form are required before material can be shipped.

EMMA conditions
Legally binding conditions for the transfer

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C57BL/6NTac-Ehd1tm1a(EUCOMM)Wtsi/WtsiBiat