B6Brd;B6N-Tyr^c-Brd Cpap^{tm1a(EUCOMM)Wtsi}/WtsiBiat

Status	Available to order
EMMA ID	EM:05725
Citation information	RRID:IMSR_EM:05725 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information.
International strain name	B6Brd;B6N-Tyr^c-Brd Cpap^{tm1a(EUCOMM)Wtsi}/WtsiBiat
Alternative name	EPD0028_7_G05
Strain type	Targeted Mutant Strains
Allele/Transgene symbol	Cpap^{tm1a(EUCOMM)Wtsi}
Gene/Transgene symbol	Cpap
Disclaimer	Please note that for EUCOMM and KOMP-CSD mice supplied to the scientific community by INFRAFRONTIER/EMMA: We can not guarantee a null mutation for Knock-out first alleles (tm1a alleles, see http://www.mousephenotype.org/about-ikmc/targeting-strategies) as the critical exon has not been deleted. That the structure of the targeted mutation in the ES cells obtained from EUCOMM/KOMP to generate EUCOMM/KOMP mice is not verified by INFRAFRONTIER/EMMA. It is recommended that the recipient confirms the mutation structure. No check for determining the copy number of the targeting construct in ES cells obtained from EUCOMM/KOMP is done by INFRAFRONTIER/EMMA. The level of quality control before mice are released is to confirm the individual mouse genotype by short range PCR.

Information from provider

Provider	Wellcome Trust Sanger Institute
Provider affiliation	Wellcome Trust Sanger Institute
Genetic information	This mouse line originates from EUCOMM ES clone EPD0028_7_G05. For further details on the construction of this clone see the page at the IMPC portal.
Phenotypic information	Potential phenotyping data in the IMPC portal
References	Disruption of mouse Cenpj, a regulator of centriole biogenesis, phenocopies Seckel syndrome.;McIntyre Rebecca E, Lakshminarasimhan Chavali Pavithra, Ismail Ozama, Carragher Damian M, Sanchez-Andrade Gabriela, Forment Josep V, Fu Beiyuan, Del Castillo Velasco-Herrera Martin, Edwards Andrew, van der Weyden Louise, Yang Fengtang, null null, Ramirez-Solis Ramiro, Estabel Jeanne, Gallagher Ferdia A, Logan Darren W, Arends Mark J, Tsang Stephen H, Mahajan Vinit B, Scudamore Cheryl L, White Jacqueline K, Jackson Stephen P, Gergely Fanni, Adams David J, ;2012;PLoS genetics;8;e1003022; 23166506 Genome-wide generation and systematic phenotyping of knockout mice reveals new roles for many genes.;White Jacqueline K, Gerdin Anna-Karin, Karp Natasha A, Ryder Ed, Buljan Marija, Bussell James N, Salisbury Jennifer, Clare Simon, Ingham Neil J, Podrini Christine, Houghton Richard, Estabel Jeanne, Bottomley Joanna R, Melvin David G, Sunter David, Adams Niels C, null null, Tannahill David, Logan Darren W, Macarthur Daniel G, Flint Jonathan, Mahajan Vinit B, Tsang Stephen H, Smyth Ian, Watt Fiona M, Skarnes William C, Dougan Gordon, Adams David J, Ramirez-Solis Ramiro, Bradley Allan, Steel Karen P, ;2013;Cell;154;452-64; 23870131

Information from EMMA

Archiving centre	University of Veterinary Medicine, Vienna, Austria

Disease and phenotype information

MGI allele-associated human disease models

Seckel syndrome / DOID:0050569

Literature references

Disruption of mouse Cenpj, a regulator of centriole biogenesis, phenocopies Seckel syndrome.;McIntyre Rebecca E, Lakshminarasimhan Chavali Pavithra, Ismail Ozama, Carragher Damian M, Sanchez-Andrade Gabriela, Forment Josep V, Fu Beiyuan, Del Castillo Velasco-Herrera Martin, Edwards Andrew, van der Weyden Louise, Yang Fengtang, null null, Ramirez-Solis Ramiro, Estabel Jeanne, Gallagher Ferdia A, Logan Darren W, Arends Mark J, Tsang Stephen H, Mahajan Vinit B, Scudamore Cheryl L, White Jacqueline K, Jackson Stephen P, Gergely Fanni, Adams David J, ;2012;PLoS genetics;8;e1003022; 23166506
Genome-wide generation and systematic phenotyping of knockout mice reveals new roles for many genes.;White Jacqueline K, Gerdin Anna-Karin, Karp Natasha A, Ryder Ed, Buljan Marija, Bussell James N, Salisbury Jennifer, Clare Simon, Ingham Neil J, Podrini Christine, Houghton Richard, Estabel Jeanne, Bottomley Joanna R, Melvin David G, Sunter David, Adams Niels C, null null, Tannahill David, Logan Darren W, Macarthur Daniel G, Flint Jonathan, Mahajan Vinit B, Tsang Stephen H, Smyth Ian, Watt Fiona M, Skarnes William C, Dougan Gordon, Adams David J, Ramirez-Solis Ramiro, Bradley Allan, Steel Karen P, ;2013;Cell;154;452-64; 23870131
Cenpj Regulates Cilia Disassembly and Neurogenesis in the Developing Mouse Cortex.;Ding Wenyu, Wu Qian, Sun Le, Pan Na Clara, Wang Xiaoqun, ;2019;The Journal of neuroscience : the official journal of the Society for Neuroscience;39;1994-2010; 30626697
Loss of the centrosomal protein Cenpj leads to dysfunction of the hypothalamus and obesity in mice.;Ding Wenyu, Zhang Changjiang, Wang Baisong, Zhou Xin, Sun Le, Zhong Suijuan, Liu Jing, Zhang Junjing, Wang Xiaoqun, Wu Qian, ;2021;Science China. Life sciences;64;419-433; 32803714
Cenpj/CPAP regulates progenitor divisions and neuronal migration in the cerebral cortex downstream of Ascl1.;Garcez Patricia P, Diaz-Alonso Javier, Crespo-Enriquez Ivan, Castro Diogo, Bell Donald, Guillemot François, ;2015;Nature communications;6;6474; 25753651

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Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

Frozen embryos. Delivered in 4 weeks (after paperwork in place). €1740^*
Rederivation of mice from frozen stock, delivery time available upon request . €3880^*
LacZ tagged null allele (tm1b allele), service fee and delivery time available upon request
Conditional ready allele (tm1c allele), service fee and delivery time available upon request
Reporterless null allele (tm1d allele), service fee and delivery time available upon request

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

^* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Genotyping protocol

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
Distribution of this strain is subject to a provider MTA. Both signing of the MTA and submission of the online EMMA Mutant Request Form are required before material can be shipped.

EMMA conditions
Legally binding conditions for the transfer

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B6Brd;B6N-Tyrc-Brd Cpaptm1a(EUCOMM)Wtsi/WtsiBiat