(C57BL/6J x B10.Cg-Trap1atm1Jvde H2d)F1/Orl
| Status | Available to order |
| EMMA ID | EM:05734 |
| International strain name | (C57BL/6J x B10.Cg-Trap1atm1Jvde H2d)F1/Orl |
| Alternative name | pKOi-P1A3 (Trap1a |
| Strain type | Targeted Mutant Strains : Conditional mutation |
| Allele/Transgene symbol | Trap1atm1Jvde, |
| Gene/Transgene symbol | Trap1a |
Information from provider
| Provider | Anne-Marie Schmitt-Verhulst |
| Provider affiliation | Centre D'immunologie Marseille-Luminy |
| Additional owner | Benoît Van den Eynde, Ludwig Institute for Cancer Research and de Duve Institute, Universite´ catholique de Louvain, Brussels, Belgium |
| Genetic information | Conditional KO of gene Trap1 due to insertion of loxP sites, one into the Acc65I site located 15 bp upstream of the ATG in the first exon, and the other into the PstI site located in the first intron. |
| Phenotypic information | Trap1 is mouse cancer-germline gene P1A encoding antigenic peptide P1A3543 presented by H-2Ld. It is expressed in testis, placenta and a subpopulation of thymic medullary epithelial cells. Both male and female Trap1/P1A-KO mice were fertile. Compared to wild type mice, Trap1/P1A-KO mice generated a slightly higher frequency of P1A3543 specific CD8 T cells in response to P1A-expressing tumors and showed increased capacity to reject spontaneously P1A-expressing tumors. |
| Breeding history | Backcrossed to B10.D2/nOlaHsd (Harlan) for more than 10 generations. |
| References |
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| Homozygous fertile | yes |
| Homozygous viable | yes |
| Homozygous matings required | no |
| Immunocompromised | no |
Information from EMMA
| Archiving centre | Institut de Transgenose, INTRAGENE, Orléans, France |
| Animals used for archiving | heterozygous 0, wild-type C57BL/6J |
| Breeding at archiving centre | Hemizygous males |
Literature references
- Minimal tolerance to a tumor antigen encoded by a cancer-germline gene.;Huijbers Ivo J, Soudja Saïdi M, Uyttenhove Catherine, Buferne Michel, Inderberg-Suso Else-Marit, Colau Didier, Pilotte Luc, Powis de Tenbossche Céline G, Chomez Patrick, Brasseur Francis, Schmitt-Verhulst Anne-Marie, Van den Eynde Benoît J, ;2012;Journal of immunology (Baltimore, Md. : 1950);188;111-21; 22140254